The clonal composition of biramous and uniramous arthropod limbs
The clonal composition of biramous and uniramous arthropod limbs
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DOI：
10.1098/rspb.2007.1327
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发表时间：
2008-05
期刊：
影响因子：
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通讯作者：
Carsten Wolff;G. Scholtz
中科院分区：
文献类型：
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作者：
Carsten Wolff;G. Scholtz
我们提出了第一个比较细胞谱系分析的uniramous和biramous四肢的节肢动物，甲壳动物Orchestia cavimana。通过单细胞标记的细胞参与肢体发育，我们能够提出第一个完整的克隆组成的节肢动物肢体。我们表明，甲壳类肢的两个主要分支，外足类和内足类，是由主肢轴的生长区的次级细分形成的。额外的肢体生长，如exites的结果，建立新的轴。与一般的看法相反，Orchestia的单枝肢不是由外足动物的丧失形成的，而是由外足动物和内足动物分裂的抑制形成的。我们的研究结果提供了一个发展的方法来区分不同种类的分支节肢动物的附属物。这导致的结论是，一个“真正的”biramous肢体包括一个内足类和一个外足类可能发生在真节肢动物的进化比以前认为的要晚得多，可能是在血统的下颌或Tetraconata。
We present the first comparative cell lineage analysis of uniramous and biramous limbs of an arthropod, the crustacean Orchestia cavimana. Via single cell labelling of the cells that are involved in limb development, we are able to present the first complete clonal composition of an arthropod limb. We show that the two main branches of crustacean limbs, exopod and endopod, are formed by a secondary subdivision of the growth zone of the main limb axis. Additional limb outgrowths such as exites result from the establishment of new axes. In contrast to general belief, uniramous limbs in Orchestia are not formed by the loss of the exopod but by suppression of the split into exopod and endopod. Our results offer a developmental approach to discriminate between the different kinds of branches of arthropod appendages. This leads to the conclusion that a ‘true’ biramous limb comprising an endopod and an exopod might have occurred much later in euarthropod evolution than has previously been thought, probably either in the lineage of the Mandibulata or that of the Tetraconata.