The complete mitochondrial DNA sequence of the short mackerel (Rastrelliger brachysoma), and its phylogenetic position within Scombroidei, Perciformes
The complete mitochondrial DNA sequence of the short mackerel (Rastrelliger brachysoma), and its phylogenetic position within Scombroidei, Perciformes
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DOI：
10.3109/19401731003622529
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发表时间：
2010-04-01
影响因子：
--
通讯作者：
Karinthanyakit, Wirangrong
中科院分区：
文献类型：
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作者：
Jondeung, Amnuay;Karinthanyakit, Wirangrong
为了支持泰国湾短体鲭鱼种群遗传结构和系统发育关系的研究，利用部分克隆技术、长PCR技术和引物步移测序技术对泰国湾短体鲭鱼（Rastrelliger brachysoma）的线粒体基因组进行了测定。线粒体基因组全长16,539 bp，包含37个线粒体基因（13个蛋白质编码基因、2个核糖体RNA基因和22个转运RNA基因）和一个控制区（CR）。在845 bp的CR中，我们鉴定了几个保守的基序。基于对应于cytb和nd 2线粒体基因的两个核苷酸数据集通过贝叶斯分析获得的遗传学强有力地支持R.鲭科中鲭族单系族中的短体。所获得的系统发育也揭示了高统计支持存在两个不同的群体，表明Scombroidei和Xiphioidei是两个独立的亚目。
In order to support studies of short mackerel population genetic structure in the Gulf of Thailand and phylogenetic relationships, the mitochondrial genome of the short mackerel, Rastrelliger brachysoma, has recently been determined by a partial cloning technique, long PCR with three pairs of newly designed primers and primer walking sequencing. The complete mitochondrial genome is 16,539 bp in length and contains 37 mitochondrial genes (13 protein-coding genes, 2 ribosomal RNA genes and 22 transfer RNA genes) and a control region (CR) as in other bony fishes. Within the 845-bp CR, we identified several conserved motifs. The phylogeny obtained by Bayesian analyses based on two nucleotide datasets corresponding to the cytb and nd2 mitochondrial genes strongly support the inclusion of R. brachysoma within the monophyletic tribe of Scombrini in the family Scombridae. The obtained phylogeny also reveals high-statistical support for the existence of two distinct groups indicating that Scombroidei and Xiphioidei are two separate suborders.