COMPRESSION LOADING INVITRO REGULATES PROTEOGLYCAN SYNTHESIS BY TENDON FIBROCARTILAGE
COMPRESSION LOADING INVITRO REGULATES PROTEOGLYCAN SYNTHESIS BY TENDON FIBROCARTILAGE
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DOI：
10.1016/0003-9861(92)90127-i
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发表时间：
1992-10-01
影响因子：
3.9
通讯作者：
VOGEL, KG
中科院分区：
文献类型：
--
作者：
KOOB, TJ;CLARK, PE;VOGEL, KG
在体外评估机械负荷对纤维软骨组织蛋白多糖合成的调节。牛肌腱纤维软骨盘每天加载无限制的，循环的，单轴压缩（5 s/min，20 min/天）和大，小蛋白多糖的合成进行了测量，通过掺入[35 S]硫酸盐。所有光盘合成主要是大蛋白聚糖时，第一次放置在文化。在培养2周后，未加载的盘主要合成小蛋白聚糖，而加载的盘继续主要产生大蛋白聚糖。35 S-标记的蛋白多糖的营业额没有显着改变的压缩制度。增加合成的大蛋白聚糖诱导4天的压缩制度后，21天的文化没有压缩。在压缩过程中包含细胞松弛素B模拟了这种诱导。放射自显影显示，细胞增殖是最小的，仅限于椎间盘边缘，而35 S标记的蛋白多糖合成发生在整个椎间盘。这些实验表明，机械压缩可以调节纤维软骨中不同蛋白多糖类型的合成。
The regulation of proteoglycan synthesis in a fibrocartilaginous tissue by mechanical loading was assessedin vitro. Discs of bovine tendon fibrocartilage were loaded daily with unconfined, cyclic, uniaxial compression (5 s/min, 20 min/day) and the synthesis of large and small proteoglycans was measured by incorporation of [35S]sulfate. All discs synthesized predominantly large proteoglycan when first placed in culture. After 2 weeks in culture nonloaded discs synthesized predominantly small proteoglycans whereas loaded discs continued to produce predominantly large proteoglycan. The turnover of35S-labeled proteoglycan was not significantly altered by the compression regime. Increased synthesis of large proteoglycans was induced by a 4-day compression regime following 21 days of culture without compression. Inclusion of cytochalasin B during compression mimicked this induction. Autoradiography demonstrated that cell proliferation was minimal and confined to the disc edges whereas35S-labeled proteoglycan synthesis occurred throughout the discs. These experiments demonstrate that mechanical compression can regulate synthesis of distinct proteoglycan types in fibrocartilage.