Enhanced healing of full-thickness burn wounds using di-rhamnolipid
Enhanced healing of full-thickness burn wounds using di-rhamnolipid
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DOI：
10.1016/j.burns.2005.07.004
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发表时间：
2006-02-01
期刊：
影响因子：
2.7
通讯作者：
Pijac, G
中科院分区：
文献类型：
--
作者：
Stipcevic, T;Pijac, A;Pijac, G
本研究的目的是研究鼠李糖脂[α - l-鼠李糖pyranosyl-(1-2)- α - l-鼠李糖pyranosyl-3-hydroxydecanoyl-3-hydroxydecanoic acid，也被称为鼠李糖脂BAC-3]在皮肤伤口愈合过程中的作用。将双鼠甘脂加入到eucerin软膏中，局部涂抹于正常sd大鼠全层烧伤创面，创面面积占体表总面积的5%。在45天内测量伤口愈合率。在显微镜下，通过对双鼠李糖脂治疗第45天结束时伤口活检的Verhoeff染色组织病理学切片进行密度分析，评估胶原含量。采用Swiss-Webster小鼠皮下多剂量试验评估鼠李糖脂的毒性。局部应用0.1%鼠李糖脂治疗全层烧伤创面，与对照组相比，治疗第21天创面愈合速度加快32% （p < 0.05）。在第35天，用0.1%鼠李糖脂软膏治疗的所有动物伤口愈合，而对照组的一些大鼠在第35天甚至第45天伤口裂开。组织学比较显示，与对照组相比，双鼠李糖脂显著降低烧伤创面胶原含量（47.5%,p < 0.05）。鼠李糖脂耐受性良好。这项研究的结果提高了双鼠李糖脂在加速正常伤口愈合和克服慢性伤口愈合失败相关缺陷方面的潜在功效的可能性。(c) 2005爱思唯尔有限公司和ISBI。版权所有。
The aim of this study was to investigate the properties of di-rhamnolipid [alpha-L-rhamnopyranosyl-(1-2)-alpha-L-rhamnopyranosyl-3-hydroxydecanoyl-3-hydroxydecanoic acid, also referred to as di-rhamnolipid BAC-3] relating to the process of cutaneous wound healing. Dirhamnolipid was prepared in a eucerin ointment and applied topically on full-thickness burn wounds in normal Sprague-Dawley rats covering 5% of the total body surface area. The rate of wound closure was measured over the period of 45 days. The collagen content was evaluated microscopically, by performing densitometric analysis on Verhoeff's stained histopathological slides of wound biopsies taken at the end of 45th day of di-rhamnolipid treatment. Di-rhamnolipid toxicity was assessed with the subcutaneous multi-dose study in Swiss-Webster mice. The treatment of full-thickness-burn wounds with topical 0.1% di-rhamnolipid accelerated the closure of wounds on day 21 of the treatment by 32% compared to the control (p < 0.05). On day 35, the wounds closed in all animals-treated with 0.1% di-rhamnolipid ointment while some rats in the control group had open wounds on days 35 and even 45. Histologic comparisons have shown that di-rhamnolipid significantly decreased collagen content in burn wounds (47.5%, p < 0.05) as compared to the vehicle-treated (control) wounds. Di-rhamnolipid was well-tolerated. The results of this study raise the possibility of potential efficacy of di-rhamnolipid in accelerating normal wound healing and perhaps in overcoming defects associated with healing failure in chronic wounds. (c) 2005 Elsevier Ltd and ISBI. All rights reserved.