The cysteine-rich region of a baculovirus VP91 protein contributes to the morphogenesis of occlusion bodies.
The cysteine-rich region of a baculovirus VP91 protein contributes to the morphogenesis of occlusion bodies.
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DOI：
10.1016/j.virol.2019.06.016
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发表时间：
2019-09
期刊：
影响因子：
3.7
通讯作者：
F. Zhou;W. Kuang;Xi Wang;Dianhai Hou;Huachao Huang;Xiulian Sun;F. Dèng;Huálín Wáng;Monqiue M van Oer
中科院分区：
文献类型：
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作者：
F. Zhou;W. Kuang;Xi Wang;Dianhai Hou;Huachao Huang;Xiulian Sun;F. Dèng;Huálín Wáng;Monqiue M van Oer
据报道，杆状病毒核心基因p91对于核衣壳组装和口腔感染是必需的。本文通过分析棉铃虫核型多角体病毒（HelicoverpaarmigeraNucleopolyhedrovirus，HearNPV）中vp 91的同源基因ha 76，研究了vp 91的功能。HA 76在HearNPV感染后期表达，ha 76的缺失表明该基因是芽殖病毒产生所必需的。构建了一系列具有截短ha 76的重组子，并进行了体外和体内分析。结果表明，HA 76的C端编码区是核衣壳组装所必需的，而N端富含半胱氨酸的区域负责口腔感染。电子显微镜分析进一步表明，富含半胱氨酸的区域有助于闭塞体（OBs）的形态发生，HA 76的氨基酸136-223对于该功能至关重要。结果揭示了VP 91的新功能，并表明对OB形态发生的影响部分与口腔感染性有关。
The baculovirus core genevp91 has been reported to be essential for nucleocapsid assembly and oral infection. Here, we studied the function ofvp91 by analyzing its homologue,ha76, in Helicoverpa armigera nucleopolyhedrovirus (HearNPV). HA76 was expressed at the late stage of HearNPV infection; deletion ofha76showed that the gene is required for budded virus production. A series of recombinants with truncatedha76was constructed and analyzedin vitroandin vivo. The results showed that the region encoding the C-terminus of HA76 was essential for nucleocapsid assembly, whereas the N-terminal cysteine-rich region was responsible for oral infection. Electron microscope analyses further showed that the cysteine-rich region contributed to morphogenesis of occlusion bodies (OBs), with amino acids 136–223 of HA76 being critical for this function. The results revealed a novel function of VP91 and suggested that the impact on OB morphogenesis is partially related to oral infectivity.