Chitosan/calcium phosphates nanosheet as a vaccine carrier for effective cross-presentation of exogenous antigens
Chitosan/calcium phosphates nanosheet as a vaccine carrier for effective cross-presentation of exogenous antigens
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壳聚糖/磷酸钙纳米片作为疫苗载体有效交叉呈递外源抗原
DOI：
10.1016/j.carbpol.2019.115172
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发表时间：
2019-11-15
影响因子：
11.2
通讯作者：
Sun, Hongfan
中科院分区：
文献类型：
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作者：
Pei, Mengyue;Liang, Jiayi;Sun, Hongfan
将改性壳聚糖与磷酸盐缓冲液（PBS）和氯化钙（CaCl 2）直接混合，制备了壳聚糖/磷酸钙纳米片作为抗原载体。具体而言，用邻苯二酚基团对壳聚糖进行改性，提高了其在中性条件下的水溶性。然后，通过混合改性壳聚糖和PBS，然后加入CaCl 2形成纳米片。在其制备过程中，抗原通过共沉淀被捕获到纳米片中。由CaHPO 4中心点2 H（2）O晶体组成的纳米片通过巨胞饮作用被树突状细胞（DC）内化，具有增强的效率。用细胞内Lysotracker和Lamp-1染色观察到纳米片诱导的抗原内体逃逸。此外，DC活化被纳米片沿着上调的共刺激标记物的表达和Th 1型细胞因子的产生触发。更重要的是，与游离抗原相比，由纳米片实现的抗原的交叉呈递显著增加。因此，壳聚糖/磷酸钙纳米片可作为疫苗载体，有效地交叉呈递外源性抗原。
Chitosan/calcium phosphate nanosheet as a promising antigen carrier was prepared via the direct mixture of modified chitosan, PBS and CaCl2. Specifically, chitosan was modified with catechol groups, and its water solubility under neutral conditions was improved. Then, nanosheet was formed by mixing modified chitosan and PBS followed by addition of CaCl2. Antigen was entrapped into the nanosheet via coprecipitation during its preparation. The nanosheet composed of CaHPO4 center dot 2H(2)O crystals, was internalized by dendritic cells (DCs) via macropinocytosis with enhanced efficiency. The antigen endosomal escape induce by nanosheet was successful observed with intracellular Lysotracker and Lamp-1 staining. Moreover, DCs activation was triggered by the nanosheet along with the up-regulated expression of co-stimulation marker and production of Th1-type cytokines. More importantly, cross-presentation of antigens achieved by the nanosheet was markedly increased when compared to free antigen. Therefore, chitosan/calcium phosphate nanosheet could be used as a vaccine carrier for effective cross-presentation of exogenous antigens.