Toluidine blue-mediated photoinactivation of periodontal pathogens from supragingival plaques
Toluidine blue-mediated photoinactivation of periodontal pathogens from supragingival plaques
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DOI：
10.1007/s10103-007-0454-x
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发表时间：
2008-01-01
影响因子：
2.1
通讯作者：
Zhang, Zhiguo
中科院分区：
文献类型：
--
作者：
Qin, Yanli;Luan, Xiuling;Zhang, Zhiguo
本研究旨在评估甲苯胺蓝（TB）介导的光动力学灭活牙周病患者牙周致病菌（PP）的效果。使用TB和635 nm激光照射进行光动力学疗法（PDT）。评价杀菌效果，并确定重要的PDT参数，包括光强度、能量剂量和TB浓度。我们的研究结果表明，TB介导的致死光敏PP在体内是可能的。然而，为了获得理想的杀菌效果，在体内治疗时需要比它们的非质子对应物更高的光和光敏剂剂量。TB 1 mg/ml联合12 J/cm ~（2+）、159 mW/cm ~（2+）光照射治疗效果最好。此外，由于相当大的个体间差异的细菌种群，TB介导的PDT可能不会同样有效的牙周病患者，并需要进一步研究，以改善这种治疗方式。
This study aimed to assess the effect of toluidine blue (TB)-mediated photodynamic inactivation of periodontal pathogens (PP) from periodontopathic patients. Photodynamic therapy (PDT) was carried out using TB and 635 nm laser light irradiation. The bactericidal effect was evaluated, and important PDT parameters including light intensity, energy dose, and TB concentration were determined. Our findings suggest that TB-mediated lethal photosensitization of PP in vivo is possible. However, to obtain ideal bactericidal effect, higher doses of light and photosensitizer should be required in treatment in vivo than their planktonic counterparts. The best therapeutic effect was observed in treatment by 1 mg/ml TB combined with 12 J/cm(2) stop at 159 mW/cm(2) stop light irradiation. Moreover, because of the considerable interindividual differences of bacterial populations, TB-mediated PDT might not be equally effective among periodontopathic patients, and further studies on improvement of this therapeutic modality is needed.