Origin of the West Nile virus responsible for an outbreak of encephalitis in the northeastern United States
Origin of the West Nile virus responsible for an outbreak of encephalitis in the northeastern United States
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DOI：
10.1126/science.286.5448.2333
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发表时间：
1999-12-17
期刊：
影响因子：
56.9
通讯作者：
Gubler, DJ
中科院分区：
文献类型：
--
作者：
Lanciotti, RS;Roehrig, JT;Gubler, DJ
1999年夏末，美国东北部爆发了人类脑炎，同时乌鸦(乌鸦)大量死亡，同一地区一个动物园的几只外来鸟类死亡。从一只死亡的智利火烈鸟脑中分离出的黄病毒的完整基因组序列，以及从包括蚊子和两个致命的人类病例在内的其他几个物种扩增的包膜糖蛋白(E-糖蛋白)基因的部分序列分析，揭示了西尼罗河病毒(WN)在自然传播周期中传播，并导致人类疾病。用E-糖蛋白特异性单抗进行抗原性定位和E-糖蛋白系统发育分析，证实这些病毒为WN。这种北美的WN病毒与1998年从以色列一只死鹅身上分离的WN病毒关系最密切。
In Late summer 1999, an outbreak of human encephalitis occurred in the northeastern United States that was concurrent with extensive mortality in crows (Corvus species) as well as the deaths of several exotic birds at a zoological park in the same area. Complete genome sequencing of a flavivirus isolated from the brain of a dead Chilean flamingo (Phoenicopterus chilensis), together with partial sequence analysis of envelope glycoprotein (E-glycoprotein) genes amplified from several other species including mosquitoes and two fatal human cases, revealed that West Nile (WN) virus circulated in natural transmission cycles and was responsible for the human disease. Antigenic mapping with E-glycoprotein-specific monoclonal antibodies and E-glycoprotein phylogenetic analysis confirmed these viruses as WN. This North American WN virus was most closely related to a WN virus isolated from a dead goose in Israel in 1998.