MALAT1 regulates osteogenic differentiation of human periodontal ligament stem cells through mediating miR-155-5p/ETS1 axis
MALAT1 regulates osteogenic differentiation of human periodontal ligament stem cells through mediating miR-155-5p/ETS1 axis
复制标题
MALAT1通过介导miR-155-5p/ETS1轴调节人牙周膜干细胞成骨分化
DOI：
10.1016/j.tice.2021.101619
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发表时间：
2021-08-14
期刊：
影响因子：
2.6
通讯作者：
Zhang, Xiaohong
中科院分区：
文献类型：
--
作者：
Hua, Li;Zhang, Xiaohong
背景：人牙周膜干细胞的成骨分化是牙周炎牙周再生治疗的关键。本研究探讨了lncRNA相关的成骨分化的机制。方法：从人牙周膜组织中提取人牙周膜干细胞，采用流式细胞仪进行鉴定。在使用成骨细胞诱导条件培养基诱导成骨分化三周后，用siRNA-MALAT 1或miR-155- 5 p抑制剂转染人牙周膜干细胞。碱性磷酸酶染色观察人牙周膜干细胞成骨情况，茜素红染色观察矿化结节形成情况。免疫印迹和qRT-PCR检测Runt相关基因2、1型胶原和骨钙素的表达。结果如下：MALAT 1表达与miR-155- 5 p表达呈负相关，miR-155- 5 p表达在人牙周膜干细胞分化中随时间下降。MALAT 1可与miR-155- 5 p结合，E26转化特异性基因1（ETS 1）是miR-155- 5 p的靶基因。在人牙周膜干细胞分化过程中，沉默MALAT 1可抑制ALP活性和矿化结节形成，并抑制runt相关基因2、胶原-1和骨钙素的表达，而miR-155- 5 p抑制剂可逆转si-MALAT 1对人牙周膜干细胞分化的影响。结论：MALAT 1通过调控miR 155 - 5 p调控人牙周膜干细胞的分化。
Background: Osteogenic differentiation of human periodontal ligament stem cells is essential to periodontal regeneration treatment for periodontitis. This study investigated the mechanism of lncRNAs-related osteogenic differentiation. Methods: Human periodontal ligament stem cells were extracted from human periodontal ligament and identified via flow cytometry. After being induced into osteogenic differentiation for three weeks using osteoblast inducing conditional media, human periodontal ligament stem cells were transfected with siRNA-MALAT1, or miR-155-5p inhibitor. Human periodontal ligament stem cells osteogenesis was observed by alkaline phosphatase staining, followed by alizarin red staining for evaluating mineralized nodes formation. Runt-related gene 2, collagen-1 and osteocalcin expressions were assessed by western blot and qRT-PCR. Results: MALAT1 expression was assumed a negative correlation with miR-155-5p expression which was dropping over time in differentiating human periodontal ligament stem cells. MALAT1 could bind with miR-155-5p, and E26 transformation specific-1 (ETS1) was the targeted gene of miR-155-5p. Silenced MALAT1 suppressed ALP activity and mineralized nodes formation and inhibited the expressions of runt-related gene 2, collagen-1 and osteocalcin in differentiating human periodontal ligament stem cells, while miR-155-5p inhibitor reversed the effect of si-MALAT1 on differentiation of human periodontal ligament stem cells. Conclusion: MALAT1 modulated differentiation of human periodontal ligament stem cells via regulating miR155-5p.