Gene expression profiling of white adipose tissue reveals paternal transmission of proneness to obesity.
Gene expression profiling of white adipose tissue reveals paternal transmission of proneness to obesity.
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DOI：
10.1038/srep21693
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发表时间：
2016-02-12
影响因子：
4.6
通讯作者：
Hatada I
中科院分区：
文献类型：
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作者：
Morita S;Nakabayashi K;Kawai T;Hayashi K;Horii T;Kimura M;Kamei Y;Ogawa Y;Hata K;Hatada I
此前，我们发现C57 BL/6 J（B6）小鼠比PWK小鼠更容易患肥胖症。此外，我们分析了这些小鼠之间的相互杂交，发现具有B6父亲的（PWK × B6）F1小鼠比具有B6母亲的（B6 × PWK）F1小鼠更容易发生饮食性肥胖。这些结果表明，饮食引起的肥胖症是父系传播。在这项研究中，我们使用下一代测序技术对B6、PWK、（PWK × B6）F1和（B6 × PWK）F1小鼠的脂肪组织进行了转录组分析。我们发现饮食诱导的肥胖症的父系传递与脂肪组织炎症、金属离子转运和纤毛相关的基因有关。此外，我们分析了在白色脂肪组织（WAT）和肥胖中表达的印记基因。父系印记基因（PEG）表达与体重呈负相关，而母系印记基因（MEGs）表达与体重呈正相关。在肥胖倾向的B6小鼠中，PEG的表达被高脂饮食下调，这表明PEG的异常低表达有助于B6小鼠中高脂饮食诱导的肥胖。此外，使用B6和PWK之间不同的单核苷酸多态性，我们确定了WAT中的候选印迹基因。
Previously, we found that C57BL/6J (B6) mice are more prone to develop obesity than PWK mice. In addition, we analyzed reciprocal crosses between these mice and found that (PWK × B6) F1 mice, which have B6 fathers, are more likely to develop dietary obesity than (B6 × PWK) F1 mice, which have B6 mothers. These results suggested that diet-induced obesity is paternally transmitted. In this study, we performed transcriptome analysis of adipose tissues of B6, PWK, (PWK × B6) F1, and (B6 × PWK) F1 mice using next-generation sequencing. We found that paternal transmission of diet-induced obesity was correlated with genes involved in adipose tissue inflammation, metal ion transport, and cilia. Furthermore, we analyzed the imprinted genes expressed in white adipose tissue (WAT) and obesity. Expression of paternally expressed imprinted genes (PEGs) was negatively correlated with body weight, whereas expression of maternally expressed imprinted genes (MEGs) was positively correlated. In the obesity-prone B6 mice, expression of PEGs was down-regulated by a high-fat diet, suggesting that abnormally low expression of PEGs contributes to high-fat diet-induced obesity in B6 mice. In addition, using single-nucleotide polymorphisms that differ between B6 and PWK, we identified candidate imprinted genes in WAT.