Nanopatterned Bulk Metallic Glass Biosensors
Nanopatterned Bulk Metallic Glass Biosensors
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DOI：
10.1021/acssensors.7b00455
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发表时间：
2017-12-01
期刊：
影响因子：
8.9
通讯作者：
Kyriakides, Themis R.
中科院分区：
文献类型：
--
作者：
Kinser, Emily R.;Padmanabhan, Jagannath;Kyriakides, Themis R.
纳米掺杂作为一种表面积增强方法，有可能提高生物传感器的信号和灵敏度。铂基大块金属玻璃(Pt-BMG)是一种生物相容材料，具有良好的电学性能，可以在空气中以较低的温度进行加工，获得纳米级的非随机形貌。本文采用葡萄糖氧化酶功能化的纳米铂-BMG电极，探索非随机和高重复性的纳米比表面积增强对葡萄糖生物传感器性能的影响。通过循环伏安法(CV)和安培伏安法(AV)等电化学测试，比较了200 nm铂-BMG电极和扁平铂-BMG对照电极的性能。在2 mM到10 mM的范围内研究葡萄糖的剂量响应。200 nm铂-BMG的有效电流密度动态范围是平板BMG的10-12倍。测得纳米粒子电极的灵敏度为3.28 mA/cm(2)/mm，也比平面电极大一个数量级。这些结果表明，非随机纳米拓扑术是一种可扩展和可定制的工程工具，它可以与铂-BMG集成，以制造具有增强信号和灵敏度的生物兼容生物传感器。
Nanopatterning as a surface area enhancement method has the potential to increase signal and sensitivity of biosensors. Platinum-based bulk metallic glass (Pt-BMG) is a biocompatible material with electrical properties conducive for biosensor electrode applications, which can be processed in air at comparably low temperatures to produce nonrandom topography at the nanoscale. Work presented here employs nanopatterned Pt-BMG electrodes functionalized with glucose oxidase enzyme to explore the impact of nonrandom and highly reproducible nanoscale surface area enhancement on glucose biosensor performance. Electrochemical measurements including cyclic voltammetry (CV) and amperometric voltammetry (AV) were completed to compare the performance of 200 nm Pt-BMG electrodes vs Flat Pt-BMG control electrodes. Glucose dosing response was studied in a range of 2 mM to 10 mM. Effective current density dynamic range for the 200 nm Pt-BMG was 10-12 times greater than that of the Flat BMG control. Nanopatterned electrode sensitivity was measured to be 3.28 mu A/cm(2)/mM, which was also an order of magnitude greater than the flat electrode. These results suggest that nonrandom nanotopography is a scalable and customizable engineering tool which can be integrated with Pt-BMGs to produce biocompatible biosensors with enhanced signal and sensitivity.