Small molecules that induce cardiomyogenesis in embryonic stem cells
Small molecules that induce cardiomyogenesis in embryonic stem cells
复制标题
DOI：
10.1021/ja038950i
复制
发表时间：
2004-02-18
影响因子：
15
通讯作者：
Schultz, PG
中科院分区：
文献类型：
--
作者：
Wu, X;Ding, S;Schultz, PG
对一个大型组合化学文库进行的基于表型细胞的筛选导致鉴定出一类二氨基嘧啶化合物（cardiogenol A-D），其可以选择性地和有效地诱导小鼠胚胎干细胞（ESC）分化为心肌细胞。ESC衍生的心肌细胞显示表达多种心肌标记物，包括肌球蛋白重链、加塔-4、MEF 2和Nkx 2.5，并自发形成搏动区。这些小分子将作为有用的化学探针来研究心肌分化，并可能最终促进胚胎干细胞在心脏修复中的治疗应用。
A phenotypic cell-based screen of a large combinatorial chemical library led to the identification of a class of diaminopyrimidine compounds (cardiogenol A−D) which can selectively and efficiently induce mouse embryonic stem cells (ESCs) to differentiate into cardiomyocytes. ESC-derived cardiomyocytes were shown to express multiple cardiac muscle markers, including myosin heavy chain, GATA-4, MEF2, and Nkx2.5, and spontaneously form beating regions. Such small molecules will serve as useful chemical probes to study cardiac muscle differentiation and may ultimately facilitate the therapeutic application of ESCs for cardiac repair.