T cell activation in a murine model of asthma
T cell activation in a murine model of asthma
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DOI：
10.1152/ajplung.1996.271.3.l476
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发表时间：
1996-09-01
影响因子：
4.9
通讯作者：
Finn, PW
中科院分区：
文献类型：
--
作者：
Krinzman, SJ;DeSanctis, GT;Finn, PW
为了确定吸入抗原引起肺部炎症和支气管高反应性的机制，我们建立了一种哮喘小鼠模型。用卵清蛋白(OVA)致敏和激发BALB/c小鼠。与磷酸盐缓冲盐水(PBS)处理的小鼠相比，OVA处理的小鼠肺阻力增加，动态顺应性降低，乙酰甲胆碱反应性增强。支气管肺泡灌洗液显示中性粒细胞和嗜酸性粒细胞比例显著增加。OVA处理的小鼠的组织切片显示杯状细胞化生，以及由淋巴细胞、中性粒细胞和嗜酸性粒细胞组成的局灶性血管周围和支气管周围的浸润物。通过流式细胞仪对胸腔淋巴细胞的分析显示，CD4(+)和B细胞群均有扩张，CD4(+)T细胞上白细胞介素2受体的表达增加，表明活化程度增强。CD44在CD4(+)和CD8(+)淋巴细胞上的表达也增加，表明局部记忆细胞群扩大。这些发现支持T淋巴细胞激活在哮喘中介导过敏性肺部炎症和支气管反应性的假说。
To determine the mechanisms by which inhaled antigens produce pulmonary inflammation and bronchial hyperreactivity, we have developed a murine model of asthma. BALB/c mice are sensitized and challenged with ovalbumin (OVA). Compared with mice treated with phosphate-buffered saline (PBS), OVA-treated mice developed increased lung resistance, decreased dynamic compliance, and greater methacholine reactivity. Bronchoalveolar lavage fluid revealed significant increases in the proportion of neutrophils and eosinophils. Tissue sections of OVA-treated mice demonstrated goblet cell metaplasia and focal perivascular and peribronchial infiltrates composed of lymphocytes, neutrophils, and eosinophils. Analysis of thoracic lymphocytes via flow cytometry revealed an expansion of both CD4(+) and B cell populations, with increased expression of interleukin-2 receptor on CD4(+) T cells, indicated increased activation. There was also increased expression of CD44 on CD4(+) and CD8(+) lymphocytes, suggesting an expansion of the local memory cell population. These findings support the hypothesis that activation of T lymphocytes mediates allergic pulmonary inflammation and bronchial reactivity in asthma.