Defect of villous cytotrophoblast differentiation into syncytiotrophoblast in Down's syndrome
Defect of villous cytotrophoblast differentiation into syncytiotrophoblast in Down's syndrome
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DOI：
10.1210/jc.85.10.3700
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发表时间：
2000-10-01
影响因子：
5.8
通讯作者：
Evain-Brion, D
中科院分区：
文献类型：
--
作者：
Frendo, JL;Vidaud, M;Evain-Brion, D
合胞体滋养层（ST）是人类胎盘的主要组成部分之一，因为它参与母胎交换和分泌妊娠特异性激素。本研究的目的是阐明ST的形成和功能的21三体（唐氏综合征）。我们首先使用细胞滋养层分化为ST的体外模型。从15个三体综合征影响的胎盘（妊娠12-35周）和10个胎龄匹配的对照胎盘中分离细胞滋养层。从正常胎盘分离的体外细胞滋养层融合形成ST。这与转录水平的增加和hCG，人胎盘催乳素，胎盘GH和瘦素的分泌有关。在三体21影响的胎盘，我们观察到的缺陷（或延迟）在ST形成和显着减少这些激素的合成和分泌相比，在培养的细胞中分离出的控制年龄匹配的胎盘。这些结果通过与对照相比，在三体性影响的胎盘的总匀浆中基因表达的显著（P < 0.001）降低来证实。这些结果将有助于了解胎儿21三体的母体激素标志物和胎盘缺陷对胎儿发育的影响。
The syncytiotrophoblast (ST) is one of the major components of the human placenta, as it is involved in fete-maternal exchanges and the secretion of pregnancy-specific hormones. The aim of this study was to elucidate the formation and function of the ST in trisomy 21 (Down's syndrome). We first used the in vitro model of cytotrophoblast differentiation into ST. Cytotrophoblasts were isolated from 15 trisomy al-affected placentas (12-35 weeks gestation) and 10 gestational age-matched control placentas. In vitro cytotrophoblasts isolated from normal placenta fused to form the ST. This was associated with an increase in transcript levels and in the secretion of hCG, human placental lactogen, placental GH, and leptin. In trisomy 21-affected placentas, we observed a defect (or a delay) in ST formation and a dramatic decrease in the synthesis and secretion of these hormones compared to those in cultured cells isolated from control age-matched placentas. These results were confirmed by a significant (P < 0.001) decrease in gene expression in total homogenates of trisomy al-affected placentas compared to controls. These results will be of help in understanding the maternal hormonal markers of fetal trisomy 21 and the consequences of placental defects for fetal development.