Detection of Notch signaling molecules in ameloblastomas
Detection of Notch signaling molecules in ameloblastomas
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DOI：
10.1111/j.1600-0714.2007.00629.x
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发表时间：
2008-04-01
影响因子：
3.3
通讯作者：
Ohki, Kousuke
中科院分区：
文献类型：
--
作者：
Kumamoto, Hiroyuki;Ohki, Kousuke
背景技术背景：为了评估Notch信号在牙源性肿瘤的发生和细胞分化中的作用，Notch受体和配体的表达在成釉细胞瘤和牙胚中进行了分析。方法：9个牙胚和32个成釉细胞瘤的组织标本通过逆转录聚合酶链反应和原位杂交检测Notch 1，Notch 2，Notch 3，Delta1和Jagged1的表达。结果：Notch1、Notch2、Notch3、Delta1和Jagged1在正常牙源性组织和牙源性肿瘤组织中均有表达。在牙胚中，Notch受体表达于牙源性上皮（除内釉上皮），并且Notch配体在内釉上皮中的表达低于其他上皮成分。牙源性间充质成分与这些Notch信号分子反应较弱。成釉细胞瘤显示Notch受体和配体在中央多面体肿瘤细胞中表达。Notch2、Delta1和Jagged1在基底膜附近的肿瘤细胞中表达。Notch受体和配体的表达在成釉细胞瘤变体的角化细胞或颗粒细胞中未发现。结论：Notch受体和配体在牙胚和成釉细胞瘤中的表达提示Notch信号通路可能调控正常和肿瘤牙源性上皮细胞的分化和增殖。
BACKGROUND: To evaluate the roles of Notch signaling in the oncogenesis and cytodifferentiation of odontogenic tumors, expression of Notch receptors and ligands was analyzed in ameloblastomas as well as in tooth germs.METHODS: Tissue specimens of nine tooth germs and 32 ameloblastomas were examined by reverse transcriptase polymerase chain reaction and by in situ hybridization to determine the expression of Notch1, Notch2, Notch3, Delta1, and Jagged1. RESULTS: mRNA expression of Notch1, Notch2, Notch3, Delta1, and Jagged1 was detected in all samples of normal and neoplastic odontogenic tissues. In tooth germs, Notch receptors were expressed in odontogenic epithelium (except for inner enamel epithelium), and expression of Notch ligands was lower in inner enamel epithelium than in other epithelial components. Odontogenic mesenchymal components were weakly reactive with these Notch signaling molecules. Ameloblastomas showed expression of Notch receptors and ligands in central polyhedral neoplastic cells. Notch2, Delta1, and Jagged1 were expressed in some neoplastic cells neighboring the basement membrane. Expression of Notch receptors and ligands was not found in keratinizing cells or granular cells in ameloblastoma variants. Stromal cells were weakly reactive with these Notch signaling molecules.CONCLUSION: Expression of Notch receptors and ligands in tooth germs and ameloblastomas suggests that Notch signaling might control cell differentiation and proliferation of normal and neoplastic odontogenic epithelium.