Effects of orally administered probiotic Pediococcus acidilactici on the small and large intestine of weaning piglets.: A qualitative and quantitative micro-anatomical study
Effects of orally administered probiotic Pediococcus acidilactici on the small and large intestine of weaning piglets.: A qualitative and quantitative micro-anatomical study
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DOI：
10.14670/hh-23.651
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发表时间：
2008-06-01
影响因子：
2
通讯作者：
Domeneghini, C.
中科院分区：
文献类型：
--
作者：
Di Giancamillo, A.;Vitari, F.;Domeneghini, C.
益生菌的研究已经接近，尤其是在最近几年，由广泛不同的观点，无论是对人类和动物的使用。乳酸菌释放细菌素，其中一些可能具有益生菌的功能。本研究的目的是探讨日粮中添加益生菌乳酸片球菌对仔猪肠道、循环淋巴细胞和断奶后前42天仔猪生产性能的影响。将16头断奶的雌性仔猪分为两组：对照组（Ctr，8头）和添加乳酸片球菌组（Pa，8头）。从断奶到试验结束监测仔猪的生长。在断奶后第42天，屠宰仔猪，用苏木精/伊红染色检查回肠和盲肠的小样本，以确定结构细节。通过绒毛和隐窝测量以及GALT测量进行组织测量。采用组织化学方法对肠粘蛋白进行研究。免疫组织化学分析用于可视化增殖以及凋亡的粘膜细胞，并确定粘膜巨噬细胞和伊加产生细胞。鉴定并计数上皮内CD 8 + T淋巴细胞。通过流式细胞仪分析循环T淋巴细胞亚群。乳酸片球菌添加对处理仔猪的体重和断奶后平均日增重有积极影响。组织学检查显示，与对照组相比，Pa动物的绒毛高度和隐窝深度增加。处理仔猪显示出比对照组更多的增殖肠上皮细胞。上皮内的CD 8 + T淋巴细胞的治疗组比对照组仔猪稀少，可能与卡他性肠炎显示在后者。我们得出结论，研究的补充可能是能够保护仔猪小肠粘膜，提高局部抵抗感染的应激断奶期。
Probiotic research has been approached, above all in recent years, by widely differing points of view, both for human and animal uses. Lactic acid bacteria release bacteriocins, and some of them may function as probiotic. The aim of the present study was to investigate the effects of dietary supplementation with the probiotic Pediococcus acidilactici on the piglet intestine, on circulating lymphocytes, and on aspects of piglet performance during the first 42 days after weaning.Sixteen female piglets were at weaning assigned to two dietary groups: Control (Ctr, 8 animals) and Pediococcus acidilactici supplemented (Pa, 8 animals). Piglets' growth was monitored from weaning to the end of the trial. On day 42 post-weaning, the piglets were slaughtered and small specimens from both ileum and cecum were examined with haematoxylin/eosin staining to ascertain structural details. Histometry was performed by villi and crypts measurements, as well as GALT measurements. Histochemical analyses were performed to investigate the intestinal mucins. Immunohistochemical analyses were used to visualize proliferating as well as apoptotic mucosal cells, and to identify mucosal macrophages and IgA producing cells. Intra-epithelial CD8+ T lymphocytes were identified and counted. Subsets of circulating T lymphocytes were analyzed by flow cytometry.Pediococcus acidilactici supplementation positively influenced weight and post-weaning average daily gain of treated piglets. Histometry showed an increase in villi height and crypts depth in Pa animals in comparison with controls. Treated piglets showed a larger number of proliferating enterocytes than controls. Intra-epithelial CD8+ T lymphocytes were scarcer in treated than in control piglets, likely in relation with catarrhal enteritis shown in the latter.We conclude that the studied supplementation was possibly able to protect the piglet small intestinal mucosa, improving local resistance to infections in the stressful weaning period.