Comprehensive transcriptional profiling of the gastrointestinal tract of ruminants from birth to adulthood reveals strong developmental stage specific gene expression
Comprehensive transcriptional profiling of the gastrointestinal tract of ruminants from birth to adulthood reveals strong developmental stage specific gene expression
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反刍动物从出生到成年胃肠道的全面转录谱揭示了强烈的发育阶段特异性基因表达
DOI：
10.1101/364752
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Bush S
中科院分区：
文献类型：
--
作者：
Bush S
对新生和幼年反刍动物来说，最重要的生理挑战之一是瘤胃的发育和建立。利用我们的绵羊(Ovis Aries)高分辨率基因表达图谱中的RNA-Seq数据子集，我们首次提供了从前反刍动物到反刍动物转变过程中整个胃肠道(GI)转录的全面特征。该数据集包括来自四个不同时间点(出生、一周、8周和成年)的164个绵羊组织样本。使用网络聚类分析，我们说明了胃肠道的复杂性如何反映在组织和发育阶段特定基因表达的差异中。新生绵羊和成年绵羊在瘤胃复合体中的转录差异最显著。对年龄匹配的绵羊和山羊的三种胃肠道组织中基因表达的比较分析表明，涉及免疫和新陈代谢的基因存在物种特异性差异。这项研究通过识别与免疫、微生物识别和代谢有关的关键基因，加深了我们对前反刍动物向反刍动物转变过程中涉及的转录转录机制的理解。这些结果为未来将基因表达与发育中的胃肠道微生物定植联系起来的研究奠定了基础，并为通过确定新疗法和基因编辑的潜在靶点来提高反刍动物的效率和生产力提供了基础。
One of the most significant physiological challenges to neonatal and juvenile ruminants is the development and establishment of the rumen. Using a subset of RNA-Seq data from our high-resolution atlas of gene expression in sheep (Ovis aries) we have provided the first comprehensive characterization of transcription of the entire gastrointestinal (GI) tract during the transition from pre-ruminant to ruminant. The dataset comprises 164 tissue samples from sheep at four different time points (birth, one week, 8 weeks and adult). Using network cluster analysis we illustrate how the complexity of the GI tract is reflected in tissue- and developmental stage-specific differences in gene expression. The most significant transcriptional differences between neonatal and adult sheep were observed in the rumen complex. Comparative analysis of gene expression in three GI tract tissues from age-matched sheep and goats revealed species-specific differences in genes involved in immunity and metabolism. This study improves our understanding of the transcriptomic mechanisms involved in the transition from pre-ruminant to ruminant by identifying key genes involved in immunity, microbe recognition and metabolism. The results form a basis for future studies linking gene expression with microbial colonization of the developing GI tract and provide a foundation to improve ruminant efficiency and productivity through identifying potential targets for novel therapeutics and gene editing.