A periodic distribution of the chain length of amylopectin as revealed by high-performance anion-exchange chromatography
A periodic distribution of the chain length of amylopectin as revealed by high-performance anion-exchange chromatography
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DOI：
10.1016/0008-6215(95)00408-4
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发表时间：
1996-03-22
影响因子：
3.1
通讯作者：
Hizukuri, S
中科院分区：
文献类型：
--
作者：
Hanashiro, I;Abe, J;Hizukuri, S
采用高效阴离子交换色谱-脉冲安培检测法，在改进的条件下，对dp 80以上的支链淀粉进行基线分离，并分析了11种植物源支链淀粉的链长分布。慈姑（Sagittaria trifolia L.）变种sinensis Makino）和其它种类的支链淀粉显示出周期波，其以dp 12的间隔划分横坐标，除了食用美人蕉和山药支链淀粉的周期波，其间隔为dp 15的数量级。因此，链长分布被分成fa，dp 6-12; fb（1），dp 13-24; fb（2），dp 25-36，和fb（3），dp > 37。短支链淀粉和长支链淀粉（n）分别具有大量和少量的fa组分，并显示出淀粉颗粒的A型和B型X射线衍射。fa组分的含量在淀粉晶型的测定中起着重要的作用。
The chains of amylopectin over dp 80 were separated on baseline resolution by high-performance anion-exchange chromatography with pulsed amperometric detection under improved conditions, and the chain-length (cl) distributions of amylopectins from eleven plant sources were analyzed. The differences in amount of each chain between arrowhead (Sagittaria trifolia L. var. sinensis Makino) and other kinds of amylopectins exhibited periodic waves which divided the abscissa at intervals of dp 12, except those of edible canna and yam amylopectins, where the interval was of the order of dp 15. Accordingly, chain-length distributions were fractionated into fa, dp 6-12; fb(1), dp 13-24; fb(2), dp 25-36, and fb(3), dp > 37. Amylopectin with short and long (n) had large and small amounts of the fa fraction, respectively, and showed A and B type X-ray diffractions of starch granules. The amount of the fa fraction was suggested to play an important role in the determination of starch crystalline polymorphs.