Polarization birefringence measurements for characterizing the myocardium, including healthy, infarcted, and stem-cell-regenerated tissues
Polarization birefringence measurements for characterizing the myocardium, including healthy, infarcted, and stem-cell-regenerated tissues
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DOI：
10.1117/1.3469844
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发表时间：
2010-07-01
影响因子：
3.5
通讯作者：
Vitkin, I. Alex
中科院分区：
文献类型：
--
作者：
Wood, Michael F. G.;Ghosh, Nirmalya;Vitkin, I. Alex
心肌梗死导致心肌结构重塑，特别是心肌细胞因坏死和胶原蛋白增加而减少，并伴有瘢痕形成。干细胞再生治疗已被证明可以改变这种重塑过程，从而改善心脏功能。健康心肌组织具有高度的纤维性和各向异性，由于平行于纤维和垂直于纤维的折射率不同，它表现出光学线性双折射。因此，与梗死和治疗诱导的重构相关的心肌结构变化将改变组织表现出的各向异性。在心肌梗死后的成年大鼠心肌上测量基于偏振的线性双折射，并与接受间充质干细胞治疗的心脏进行比较。点测量和成像数据均显示梗死区域双折射减少，在干细胞再生治疗后部分反弹回健康值。这些结果证明了光学偏振法通过测量的各向异性来表征心肌微观组织状态的能力，以及这种方法监测心肌梗死再生治疗的潜力。(C) 2010光学仪器工程师学会。(DOI: 10.1117/1.3469844)
Myocardial infarction leads to structural remodeling of the myocardium, in particular to the loss of cardiomyocytes due to necrosis and an increase in collagen with scar formation. Stem cell regenerative treatments have been shown to alter this remodeling process, resulting in improved cardiac function. As healthy myocardial tissue is highly fibrous and anisotropic, it exhibits optical linear birefringence due to the different refractive indices parallel and perpendicular to the fibers. Accordingly, changes in myocardial structure associated with infarction and treatment-induced remodeling will alter the anisotropy exhibited by the tissue. Polarization-based linear birefringence is measured on the myocardium of adult rat hearts after myocardial infarction and compared with hearts that had received mesenchymal stem cell treatment. Both point measurement and imaging data show a decrease in birefringence in the region of infarction, with a partial rebound back toward the healthy values following regenerative treatment with stem cells. These results demonstrate the ability of optical polarimetry to characterize the micro-organizational state of the myocardium via its measured anisotropy, and the potential of this approach for monitoring regenerative treatments of myocardial infarction. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3469844]