Cloning and characterization of a murine SIL gene
Cloning and characterization of a murine SIL gene
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DOI：
10.1006/geno.1995.1271
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发表时间：
1995-12-10
期刊：
影响因子：
4.4
通讯作者：
Aplan, PD
中科院分区：
文献类型：
--
作者：
CollazoGarcia, N;Scherer, P;Aplan, PD
25% 的 T 细胞急性淋巴细胞白血病儿童的人类 SIL 基因因位点特异性间质缺失而被破坏。由于转录活性基因容易发生重组事件，因此这种损伤的复发性表明SIL基因产物在经历这种间质缺失的细胞类型中具有转录活性，并且SIL基因产物可能在正常淋巴发育中发挥作用。为了促进 SIL 基因功能的研究，我们克隆并表征了小鼠 SIL 基因。预测的鼠 SIL 蛋白与人类基因有 75% 的同一性，在整个开放阅读框内具有良好的同源性。体外翻译的 SIL cDNA 产生的蛋白质比预测的 139 kDa 蛋白质稍大。尽管之前的报告仅在造血组织中检测到 SIL mRNA 表达，但敏感的 RT-PCR 测定表明 SIL 表达无处不在，在所有检查的组织中均可检测到。由于 RT-PCR 测定表明 SIL mRNA 表达在快速增殖的组织中较高，因此我们使用终末分化的小鼠红白血病模型测定了 SIL mRNA 表达，发现它随着终末分化而显着降低。这些研究表明，人类SIL基因产物在啮齿类动物中相当保守，并表明SIL基因产物可能在细胞增殖中发挥作用。 (C) 1995 学术出版社
The human SIL gene is disrupted by a site-specific interstitial deletion in 25% of children with T-cell acute lymphoblastic leukemia. Since transcriptionally active genes are prone to recombination events, the recurrent nature of this lesion suggests that the SIL gene product is transcriptionally active in the cell type that undergoes this interstitial deletion and that the SIL gene product may play a role in normal lymphoid development. To facilitate studies of SIL gene function, we have cloned and characterized a murine SIL gene. The predicted murine SIL protein is 75% identical to the human gene, with good homology throughout the open reading frame. An in vitro translated SIL cDNA generated a protein slightly larger than the predicted 139-kDa protein. Although a prior report detected SIL mRNA expression exclusively in hematopoietic tissues, a sensitive RT-PCR assay demonstrated SIL expression to be ubiquitous, detectable in all tissues examined. Since the RT-PCR assay suggested that SIL mRNA expression was higher in rapidly proliferating tissues, we assayed SIL mRNA expression using a murine erythroleukemia model of terminal differentiation and found it to be dramatically decreased in conjunction with terminal differentiation. These studies demonstrate that the human SIL gene product is quite well conserved in rodents and suggest that the SIL gene product may play a role in cell proliferation. (C) 1995 Academic Press, Inc.