Effect of transferrin on hypomyelination induced by iron deficiency
Effect of transferrin on hypomyelination induced by iron deficiency
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DOI：
10.1002/jnr.21709
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发表时间：
2008-09-01
影响因子：
4.2
通讯作者：
Pasquini, J. M.
中科院分区：
文献类型：
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作者：
Badaracco, M. E.;Ortiz, E. H.;Pasquini, J. M.
我们使用大鼠缺铁模型来分析铁可用性中断对少突胶质细胞 (OLGc) 成熟和髓鞘形成的影响，并探讨在 3 日龄时颅内注射 (ICI) 脱铁转铁蛋白 (aTf) 对此过程可能产生的有益影响。出生后第 17 天和第 24 天进行的研究表明，缺铁会导致 24 天龄时髓磷脂蛋白和脂质减少。免疫组织化学显示，在未经治疗的缺铁（ID）大鼠中，与正常对照相比，抗腺瘤性息肉病（APC）和抗MBP抗体的免疫反应性显着下降，而在用aTf ICI治疗的ID大鼠中，这些标记物的免疫反应性增加。 H-铁蛋白的免疫反应性也出现了类似的情况。与正常对照或经 aTf 处理的 ID 动物制备的 OLGc 培养物相比，ID 大鼠的原代 OLGc 培养物中存在大量针对细胞表面糖蛋白 NCAM 多唾液酸化形式 (PSA-NCAM) 的抗体呈阳性的细胞。使用 aTf 治疗后，ID 大鼠培养物中的 MBP+ 细胞数量增加。用特定的抗蛋白朊病毒细胞 (PrPc) 抗体评估的脂筏的存在显示，在 ID 大鼠培养物中，PrPc 阳性结构的数量较少。用 aTf 的单一 ICI 治疗 ID 动物可刺激髓鞘形成，从而对受 ID 影响的不同生化参数产生显着校正。 (C) 2008 Wiley-Liss, Inc.
We have used a model of iron deficiency in the rat to analyze the effects of a disruption in iron availability on oligodendroglial cell (OLGc) maturation and myelinogenesis and to explore the possible beneficial influence of an intracranial injection (ICI) of apotransferrin (aTf) at 3 days of age on this process. Studies carried out on postnatal days 17 and 24 showed that iron deficiency produced a decrease in myelin proteins and lipids at 24 days of age. Immunohistochemistry showed that in untreated iron-deficient (ID) rats, the immunoreactivity of anti-adenomatous polyposis coli (APC) and anti-MBP antibodies decreased markedly with reference to normal controls, whereas in ID rats treated with an ICI of aTf, the immunoreactivity of these markers increased. A similar situation occurred with the immunoreactivity of H-ferritin. In primary OLGc cultures from ID rats, there was a high number of cells positive to the antibody against the polysialylated form of the cell surface glycoprotein NCAM (PSA-NCAM) compared with in OLGc cultures prepared from normal controls or from ID animals treated with aTf. The number of MBP+ cells in cultures from ID rats increased after treatment with aTf. The presence of lipid rafts evaluated with a specific anti-protein prion cellular (PrPc) antibody showed a smaller number of PrPc-positive structures in ID rat cultures. Treatment of the ID animals with a single ICI of aTf stimulated myelination, producing a significant correction in the different biochemical parameters affected by ID. (C) 2008 Wiley-Liss, Inc.