Influence of the allelic variants encoded at the Gli-B1 locus, responsible for a major allergen of wheat, on IgE reactivity for patients suffering from food allergy to wheat
Influence of the allelic variants encoded at the Gli-B1 locus, responsible for a major allergen of wheat, on IgE reactivity for patients suffering from food allergy to wheat
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DOI：
10.1021/jf062749k
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发表时间：
2007-02-07
影响因子：
6.1
通讯作者：
Moneret-Vautrin, Denise-Anne
中科院分区：
文献类型：
--
作者：
Denery-Papini, Sandra;Lauriere, Michel;Moneret-Vautrin, Denise-Anne
小麦呈现出重要的遗传多样性，这可能有助于寻找过敏性降低的品种。ω（5）-麦醇溶蛋白已被描述为患有小麦依赖性运动诱导的过敏反应（WDEIA）和某些慢性荨麻疹（U）病例的小麦过敏患者的主要过敏原。我们的目的是研究在Gli-B1基因座编码的ω（5）-麦醇溶蛋白的遗传变异性对这些患者的IgE抗体的反应性的影响。我们选择了在Gli-B1上表达13个等位基因的品种，包括小麦/黑麦易位，并研究了对ω（5）-麦胶蛋白特异性的兔抗血清和10名患者的IgE的麦胶蛋白的反应性。来自9名WDEIA和U患者的抗血清和IgE强烈检测到大多数Gli-B1等位基因表达的ω（5）-麦醇溶蛋白，但对从易位小麦中提取的麦醇溶蛋白和黑麦醇溶蛋白没有反应或反应微弱。选择缺乏Gli-B1基因座的基因型可能会降低小麦过敏原性。
Wheat presents an important genetic diversity that could be useful to look for cultivars with reduced allergencity. omega(5)-Gliadins have been described as major allergens for wheat allergic patients suffering from wheat-dependent exercise-induced anaphylaxis (WDEIA) and some cases of chronic urticaria (U). Our objective was to study the influence of genetic variability at the Gli-B1 locus encoding for omega(5)-gliadins on the reactivity of IgE antibodies from these patients. We selected cultivars expressing 13 alleles at Gli-B1 including a wheat/rye translocation and studied the reactivity to gliadins of a rabbit antiserum specific for omega(5)-gliadins and of IgE from 10 patients. The antiserum and IgE from nine patients with WDEIA and U strongly detected omega(5)-gliadins expressed by most of the Gli-B1 alleles but showed no or faint responses to the gliadins and secalins extracted from the translocated wheat. The selection of genotypes lacking the Gli-B1 locus may reduce wheat allergenicity.