Strategies for regeneration of the bone using porcine adult adipose-derived mesenchymal stem cells
Strategies for regeneration of the bone using porcine adult adipose-derived mesenchymal stem cells
复制标题
DOI：
10.1016/j.theriogenology.2010.11.020
复制
发表时间：
2011-05-01
期刊：
影响因子：
2.8
通讯作者：
Wheeler, M. B.
中科院分区：
文献类型：
--
作者：
Monaco, E.;Bionaz, M.;Wheeler, M. B.
骨是一种可塑性组织，具有很强的愈合能力。然而，由于疾病或创伤导致的广泛骨丢失需要组织工程应用。目前，骨移植是骨修复的金标准，但存在严重的局限性，包括供体部位发病率，排斥反应和有限的组织再生。干细胞的使用似乎是克服这些限制的一种手段。骨髓间充质干细胞（BMSC）已被选择，到目前为止，干细胞治疗骨再生。然而，研究表明，脂肪干细胞（ASC）具有与BMSC相似的免疫表型、形态学、多谱系潜能和转录组。此外，ASC更丰富，更容易获得，供体发病率更低，这可能使ASC成为BMSC的更好替代品。ASC也能够迁移到损伤部位，并具有与BMSC相似的免疫抑制能力。此外，ASC已在几个物种的体外和体内表现出广泛的成骨能力，大大增强了关键尺寸缺损的愈合。支架与ASC和生长因子的组合使用为引导骨再生提供了有价值的工具，特别是对于复杂的解剖缺损。一些关键因素包括ASC-支架相互作用和多孔骨诱导结构的适当三维设计。本文综述了为ASC用于骨再生的临床翻译提供有力支持的数据。(C)2011 Elsevier Inc. All rights reserved.
Bone is a plastic tissue with a large healing capability. However, extensive bone loss due to disease or trauma requires tissue-engineering applications. Presently, bone grafting is the gold standard for bone repair, but presents serious limitations including donor site morbidity, rejection, and limited tissue regeneration. The use of stem cells appears to be a means to overcome such limitations. Bone marrow mesenchymal stem cells (BMSC) have been the choice, thus far, for stem cell therapy for bone regeneration. However, it has been shown that adipose-derived stem cells (ASC) have similar immunophenotype, morphology, multilineage potential, and transcriptome compared to BMSC. Moreover, ASC are much more abundant, more accessible and have lower donor morbidity, which combined may make ASC a better alternative to BMSC. ASC are also able to migrate to the site of injury and have immunosuppressive abilities similar to BMSC. Further, ASC have demonstrated extensive osteogenic capacity both in vitro and in vivo in several species, greatly enhancing the healing of critical size defects. The use of scaffolds in combination with ASC and growth factors provides a valuable tool for guided bone regeneration, especially for complex anatomic defects. Some critical elements include ASC-scaffold interactions and appropriate three-dimensional design of the porous osteoinductive structures. This review examines data that provides strong support for the clinical translation of ASC for bone regeneration. (C) 2011 Elsevier Inc. All rights reserved.