Ancient DNA from Ascaris:: extraction amplification and sequences from eggs collected in coprolites
Ancient DNA from Ascaris:: extraction amplification and sequences from eggs collected in coprolites
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DOI：
10.1016/s0020-7519(01)00214-4
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发表时间：
2001-08-01
影响因子：
4
通讯作者：
Hänni, C
中科院分区：
文献类型：
--
作者：
Loreille, O;Roumat, E;Hänni, C
在比利时纳穆尔的中世纪遗址上，对粪便的分析表明，存在许多保存完好的蛔虫卵。磷灰石样品复水后，收集了104个鸡蛋，并采用基于超声波和苯酚-氯仿的方法进行提取。通过重复扩增、克隆和测序，获得了3个重叠的18S rRNA基因片段和1个细胞色素b基因片段。对这些序列的分析证实了这些虫卵来自蛔虫。我们的研究表明，粪便可能是一种有趣的寄生虫来源，可以很容易地使用分子方法来识别。对蠕虫寄生虫的古代DNA的研究很有兴趣，因为它可能回答传染病史上长期存在的问题，并为将这些古代序列与现代种群的序列进行比较提供了可能性。(C)2001年澳大利亚寄生虫学协会。爱思唯尔科学有限公司出版。保留所有权利。
On the Middle-Age site of Namur (Belgium) the analysis of coprolites revealed the presence of many well-preserved Ascaris eggs. Following rehydratation of the coprolite samples, 104 eggs were collected and extracted with an ultrasonication and phenol-chloroform based method. Three overlapping fragments of the 18S rRNA gene and one fragment of the cytochrome b gene have been reproducibly amplified, cloned and sequenced. The analysis of these sequences confirms the identification of the eggs as coming from Ascaris. Our study reveals that coprolites can be an interesting source of parasites that can be readily identified using molecular approaches. The study of ancient DNA from helminth parasites is of interest as it may answer long-standing questions in the history of infectious diseases and gives a possibility to compare these ancient sequences with those of modern populations. (C) 2001 Australian Society for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved.