RABIES VIRUS CROSS-REACTIVE MURINE T CELL CLONES ANALYSIS OF HELPER AND DELAYED-TYPE HYPERSENSITIVITY FUNCTION
RABIES VIRUS CROSS-REACTIVE MURINE T CELL CLONES ANALYSIS OF HELPER AND DELAYED-TYPE HYPERSENSITIVITY FUNCTION
复制标题
DOI：
10.1089/vim.1990.3.41
复制
发表时间：
1990-01-01
期刊：
影响因子：
2.2
通讯作者：
OSTERHAUS A
中科院分区：
文献类型：
--
作者：
BUNSCHOTEN H;DIETZSCHOLD B;OSTERHAUS A
对狂犬病病毒免疫的BALB/c小鼠的三个T细胞克隆进行了特异性和功能分析。这些克隆被证明与不同的狂犬病病毒分离株(PM、ERA、CVS、HEP)以及其他Lyssav属的代表病毒，如Duvenhage-6(DUV6)和Mokola(Mok)病毒具有广泛的交叉反应。这些克隆检测到三种不同的表位：PM、Hep、Mok和DUV6病毒共有的基质蛋白(M)上表达的表位(克隆aa8)，PM、ERA、CVS、Hep和Mok病毒共有的M蛋白上表达的表位(克隆35A)，以及PM、ERA、CVS、Hep和Mok病毒共有的糖蛋白(G蛋白)上表达的表位(克隆Bg2)。所有克隆的抗原识别均为MHC限制性的，均表现为CD4+CD8-表型。用Hep病毒皮内注射同系小鼠的T细胞静脉接种，可引起TH1细胞的局部迟发型超敏反应。在体外，这些克隆能够为狂犬病病毒诱导的B细胞提供帮助，从而产生针对狂犬病病毒所有四种结构蛋白的病毒特异性抗体。对这种抗体反应的进一步分析表明，它的一部分是针对诱导病毒中和抗体的G蛋白的抗原决定簇。
Three T cell clones derived from rabies virus-immunized BALB/c mice were analysed for specificity and function. The clones proved to be broadly cross-reactive by responding to different rabies virus isolates (PM, ERA, CVS, HEP) and other representatives of the genus Lyssavirus, like the Duvenhage-6 (DUV6) and Mokola (MOK) viruses. The clones detected three different epitopes: an epitope expressed on the matrix protein (M) shared by PM, HEP, MOK and DUV6 viruses (clone AA8), an epitope expressed on the M-protein shared by PM, ERA, CVS, HEP and MOK viruses (clone 35A) and finally an epitope expressed on the glycoprotein (G-protein) shared by PM, ERA, CVS, HEP and MOK viruses (clone BG2). Antigen recognition of all clones proved to be MHC-restricted and they all displayed the CD4+CD8- phenotype. Intravenous inoculation of the T cells in syngeneic mice, which had been injected intracutaneously in the ear with HEP virus, resulted in a localized DTH reaction characteristic for TH1 cells. In vitro, the clones were able to provide help to rabies virus-primed B cells, resulting in the production of virus-specific antibodies directed against all the four structural proteins of rabies virus. Further analysis of this antibody response revealed that part of it was directed against antigenic determinants of the G-protein which induce virus neutralizing antibody.