Toward fully synthetic N-linked glycoproteins
Toward fully synthetic N-linked glycoproteins
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DOI：
10.1002/anie.200390131
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发表时间：
2003-01-01
影响因子：
16.6
通讯作者：
Danishefsky, SJ
中科院分区：
文献类型：
--
作者：
Miller, JS;Dudkin, VY;Danishefsky, SJ
大量的完全合成的、均质的、N-连接的糖肽可以通过高度收敛和灵活的方案获得。仅需要四个转化以高产率产生天然连接的糖肽。游离聚糖的胺化之后是用肽的天冬酰胺残基酰化和Fmoc脱保护;天然化学连接完成拼图以提供N-连接的糖肽（参见方案）。Fmoc= 9-芴基甲氧基羰基。
Substantial quantities of fully synthetic, homogeneous, N-linked glycopeptides may be accessed by a highly convergent and flexible protocol. Only four transformations are required to generate naturally linked glycopeptides in high yield. Amination of a free glycan is followed by acylation with an asparagine residue of a peptide and Fmoc deprotection; native chemical ligation completes the puzzle to afford N-linked glycopeptides (see scheme). Fmoc= 9-fluorenylmethoxycarbonyl.