Early Stages of In Situ Bladder Regeneration in a Rodent Model
Early Stages of In Situ Bladder Regeneration in a Rodent Model
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DOI：
10.1089/ten.tea.2009.0697
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发表时间：
2010-08-01
影响因子：
4.1
通讯作者：
Christ, George
中科院分区：
文献类型：
--
作者：
Burmeister, David;AbouShwareb, Tamer;Christ, George
手术切除约70%的膀胱（次全膀胱[STC]）被用作模型系统，以深入了解成年哺乳动物体内的正常再生过程。雌性F344大鼠接受STC，STC后2、4和8周，通过显微计算机断层扫描、尿动力学（膀胱功能研究）药理学研究和免疫组织化学监测膀胱再生。计算机断层扫描成像显示膀胱大小在STC后2、4和8周呈时间依赖性增加，这与膀胱功能的恢复呈正相关。在研究的所有时间点，膀胱完全排空。最大的收缩反应，药理学激活和电场刺激增加随着时间的推移，在孤立的组织条再生膀胱，但保持较低的所有时间点相比，从年龄匹配的控制膀胱条。STC大鼠膀胱壁的免疫染色表明祖细胞和细胞增殖在再生反应中的作用。免疫染色和电场刺激诱导的收缩反应的存在下，证实再生膀胱的神经支配。这些初步研究确立了本模型系统用于研究体内从头组织再生的效用，并且可以提供关于优化用于临床应用的内在再生能力的指导。
Surgical removal of approximate to 70% of the bladder (subtotal cystectomy [STC]) was used as a model system to gain insight into the normal regenerative process in adult mammals in vivo. Female F344 rats underwent STC, and at 2, 4, and 8 weeks post-STC, bladder regeneration was monitored via microcomputed tomography scans, urodynamic (bladder function studies) pharmacologic studies, and immunohistochemistry. Computed tomography imaging revealed a time-dependent increase in bladder size at 2, 4, and 8 weeks post-STC, which positively correlated with restoration of bladder function. Bladders emptied completely at all time points studied. The maximal contractile response to pharmacological activation and electrical field stimulation increased over time in isolated tissue strips from regenerating bladders, but remained lower at all time points compared with strips from age-matched control bladders. Immunostaining of the bladder wall of STC rats suggested a role for progenitor cells and cellular proliferation in the regenerative response. Immunostaining and the presence of electrical field stimulation-induced contractile responses verified innervation of the regenerated bladder. These initial studies establish the utility of the present model system for studying de novo tissue regeneration in vivo and may provide guidance with respect to optimization of intrinsic regenerative capacity for clinical applications.