Partitioning of bacterial communities between seawater and healthy, black band diseased, and dead coral surfaces
Partitioning of bacterial communities between seawater and healthy, black band diseased, and dead coral surfaces
复制标题
DOI：
10.1128/aem.68.5.2214-2228.2002
复制
发表时间：
2002-05-01
影响因子：
4.4
通讯作者：
Fouke, BW
中科院分区：
文献类型：
--
作者：
Frias-Lopez, J;Zerkle, AL;Fouke, BW
在荷属安的列斯群岛南加勒比海库拉索岛感染黑带病(BBD)的三种珊瑚的表层和上覆海水中的细菌群落的组成和多样性中观察到了明显的分割。用通用保守的引物对细菌16S rRNA基因(RDNA)进行PCR扩增和测序，已鉴定出524个独特的细菌序列，隶属于12个细菌科。海水与健康、黑带病和死亡珊瑚表面的细菌群落组成的分子序列相似性不到5%。BBD细菌垫迅速迁徙并杀死珊瑚组织。从BBD垫构建的克隆文库由8个细菌区和13%的未知数组成。从受感染的珊瑚表面分离出了几个代表以前在其他海洋和陆地生物(包括人类)中发现的细菌的序列，包括梭状芽孢杆菌、弧菌、弯曲杆菌、发酵细胞、柱状细胞和毛霉。
Distinct partitioning has been observed in the composition and diversity of bacterial communities inhabiting the surface and overlying seawater of three coral species infected with black band disease (BBD) on the southern Caribbean island of Curacao, Netherlands Antilles. PCR amplification and sequencing of bacterial 16S rRNA genes (rDNA) with universally conserved primers have identified over 524 unique bacterial sequences affiliated with 12 bacterial divisions. The molecular sequences exhibited less than 5% similarity in bacterial community composition between seawater and the healthy, black band diseased, and dead coral surfaces. The BBD bacterial mat rapidly migrates across and kills the coral tissue. Clone libraries constructed from the BBD mat were comprised of eight bacterial divisions and 13% unknowns. Several sequences representing bacteria previously found in other marine and terrestrial organisms (including humans) were isolated from the infected coral surfaces, including Clostridium spp., Arcobacter spp., Campylobacter spp., Cytophaga fermentans, Cytophaga columnaris, and Trichodesmium tenue.