The Bacillus stearothermophilus replicative helicase:: cloning, overexpression and activity

The Bacillus stearothermophilus replicative helicase:: cloning, overexpression and activity
复制标题

DOI：
10.1016/s0167-4781(99)00024-x
复制
发表时间：
1999-03-19
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
影响因子：
--
通讯作者：
Wigley, DB
Wigley, DB
中科院分区：
其他
文献类型：
--
作者：
Bird, LE;Wigley, DB

文献摘要

被引文献

相似文献

作为革兰氏阳性细菌原体的生化和结构研究的一部分，我们描述了嗜热脂肪芽孢杆菌复制解旋酶DnaB的克隆。该蛋白与大肠杆菌和枯草芽孢杆菌的复制解旋酶分别有45%和82%的相同。重组DnaB被纯化并证明是一种活性解旋酶。(C) 1999 Elsevier Science B.V.版权所有
As part of biochemical and structural studies of the primosome of a Gram positive bacterial species, we describe the cloning of the Bacillus stearothermophilus replicative helicase, DnaB. The protein is 45% and 82% identical to the Escherichia coli and B. subtilis replicative helicases, respectively. Recombinant DnaB was purified and shown to be an active helicase. (C) 1999 Elsevier Science B.V. All rights reserved.