CLONING AND SEQUENCE-ANALYSIS OF CDNA-ENCODING A PRECURSOR FOR RAT-BRAIN NATRIURETIC PEPTIDE
CLONING AND SEQUENCE-ANALYSIS OF CDNA-ENCODING A PRECURSOR FOR RAT-BRAIN NATRIURETIC PEPTIDE
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DOI：
10.1016/0006-291x(89)92268-7
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发表时间：
1989-03-31
影响因子：
3.1
通讯作者：
MATSUO, H
中科院分区：
文献类型：
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作者：
KOJIMA, M;MINAMINO, N;MATSUO, H
脑钠肽（BNP）是一种新型的利钠肽，目前仅在猪脑和心房中发现。免疫学观察表明，大鼠和猪BNP可能因物种而存在结构差异。为了鉴定大鼠脑钠肽，我们构建了大鼠心房cDNA文库，并以猪脑钠肽的部分cDNA为探针，筛选了编码大鼠脑钠肽前体的克隆。通过对克隆的cDNA进行测序，推导出包含121个残基的大鼠BNP-前体的氨基酸序列，其在N-末端携带26个残基的推定信号肽，在C-末端携带与猪BNP-32同源的区域。此外，在大鼠和猪的BNP前体之间的3“-非翻译AT-丰富的区域中观察到非常高的同源性。比较目前已鉴定的ANP和BNP前体的序列，讨论BNP家族的结构和加工特征。
Brain natriuretic peptide (BNP) is a new type of natriuretic peptide, which has so far been identified only in porcine brain and atrium. Immunological observations suggest that rat and porcine BNP may have structural difference according to species. To identify rat BNP, we constructed a rat atrial cDNA library, and screened for clones encoding rat BNP-precursor by using part of porcine BNP cDNA as a probe. By sequencing a cloned cDNA, the amino acid sequence of rat BNP-precursor comprising 121 residues was deduced as carrying a 26-residue putative signal peptide at the N-terminus and a region homologous to porcine BNP-32 at the C-terminus. In addition, remarkably high homology between rat and porcine BNP-precursors was observed in the 3''-untranslated AT-rich region. Comparing sequences of precursors of ANP and BNP thus far identified, structural and processing features characteristic of the BNP family were discussed.