Acute impact of agriculture on high-affinity methanotrophic bacterial populations.
Acute impact of agriculture on high-affinity methanotrophic bacterial populations.
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DOI：
10.1111/j.1462-2920.2008.01587.x
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发表时间：
2008-07
影响因子：
5.1
通讯作者：
P. Maxfield;E. Hornibrook;R. Evershed
中科院分区：
文献类型：
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作者：
P. Maxfield;E. Hornibrook;R. Evershed
暴露于大气相关浓度的（13）CH（4）（2 ppmv），然后（13）C-磷脂脂肪酸稳定同位素探测允许评估的高亲和力甲烷细菌汇迄今无法达到的细节。利用这种方法，来自长期农业实验的无机肥料处理的土壤显示出甲烷氧化细菌细胞数量的> 70%的显着减少。土壤甲烷汇容量的减少略低于直接观察到的甲烷氧化细菌数量的减少，表明对高亲和力甲烷氧化细菌的抑制作用并不完全通过CH（4）氧化速率来表达。研究结果强调，需要严格评估普遍适用的农业做法，就其看不见的负面影响土壤微生物多样性有关的陆地汇的大气痕量气体。
Exposure of mineral soils to atmospherically relevant concentrations of (13)CH(4) (2 ppmv) followed by (13)C-phospholipid fatty acid stable isotope probing allows assessment of the high-affinity methanotrophic bacterial sink in hitherto unattainable detail. Utilizing this approach, inorganic fertilizer-treated soils from a long-term agricultural experiment were shown to display dramatic reduction, by > 70%, of the methanotrophic bacterial cell numbers. Reduction in the methane sink capacity of the soils was slightly lower than the directly observed reduction in methanotrophic bacterial counts, indicating that the inhibitory effects on high-affinity methanotrophic bacteria are not fully expressed through CH(4) oxidation rates. The results emphasize the need to rigorously assess commonly applied agricultural practices with respect to their unseen negative impacts on soil microbial diversity in relation to terrestrial sinks for atmospheric trace gases.