Newborn rabbit alveolar macrophages are deficient in two microbicidal cationic peptides, MCP-1 and MCP-2.
Newborn rabbit alveolar macrophages are deficient in two microbicidal cationic peptides, MCP-1 and MCP-2.
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新生兔肺泡巨噬细胞缺乏两种杀菌阳离子肽 MCP-1 和 MCP-2。
DOI：
10.1164/arrd.1985.132.4.901
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Lehrer,RI
中科院分区：
文献类型：
--
作者：
Ganz,T;Sherman,MP;Selsted,ME;Lehrer,RI
成年兔肺泡巨噬细胞（AM）含有2种阳离子肽，在体外对细菌、真菌和一些包膜病毒具有广谱抗菌活性。我们定性地测定了1日龄（1d）、7日龄（7d）、21日龄（21d）和成年兔AM中这两种肽的含量，发现1d AM中这两种肽都缺乏。从AM中提取的MCP-1水平相对于已知的纯化肽标准进行了定量，发现在AM的1d和21d之间增加了6倍。成年AM的MCP-1产量是1d AM的9倍，尽管每个细胞的酸提取蛋白含量几乎相同。利用免疫过氧化物酶技术，我们发现MCP-1和MCP-2的缺乏与1d AM一致，并且所有7天或以上的AM都可检测到MCP。7天大的AM（以及较小程度的1天大的AM）在细胞培养中将35s -半胱氨酸结合到细胞内MCP中，表明AM积极合成这些肽。这些抗菌物质的缺乏可能导致新生兔AM功能不成熟。
Adult rabbit alveolar macrophages (AM) contain 2 cationic peptides with a broad spectrum of antimicrobial activityin vitroagainst bacteria, fungi, and some enveloped viruses. We determined the amounts of both peptides qualitatively in 1-day-old (1d), 7-day-old (7d), 21-day-old (21d), and adult rabbit AM and found that 1d AM were deficient in both peptides. The levels of MCP-1 extractable from AM were quantitated relative to known standards of purified peptides and were found to increase 6-fold between 1d and 21d AM. Adult AM yielded 9 times as much MCP-1 as did 1d AM despite nearly the same acid-extractable protein content per cell. Using immunoperoxidase techniques we showed that the deficiency of MCP-1 and MCP-2 involves 1d AM uniformly and that all AM 7 days or older have detectable MCP. Seven-day-old AM (and to a lesser extent 1d AM) incorporated35S-cysteine into intracellular MCP in cell culture, indicating that AM actively synthesize these peptides. The deficiency of these antimicrobial substances may contribute to functional immaturity of newborn rabbit AM.