The miR172/IDS1 signaling module confers salt tolerance through maintaining ROS homeostasis in cereal crops
The miR172/IDS1 signaling module confers salt tolerance through maintaining ROS homeostasis in cereal crops
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miR172/IDS1信号模块通过维持谷类作物的ROS稳态来赋予耐盐性
DOI：
10.1111/nph.17211
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发表时间：
2021-02-18
期刊：
影响因子：
9.4
通讯作者：
Liu, Jie
中科院分区：
文献类型：
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作者：
Cheng, Xiliu;He, Qiang;Liu, Jie
盐胁迫会导致作物体内过多的活性氧积累，从而对生物组织造成严重的氧化损伤。MicroRNAs(MiRNAs)作为主调控因子，协调逆境反应调控网络和耐盐性。然而，miRNAs在调节谷类作物耐盐性，特别是在盐引发的ROS清除中的基本作用仍然很不清楚。通过小RNA测序，在水稻中发现了一个对盐响应的miR172。此外，通过构建miR172过表达或MIR172基因功能缺失突变系，明确了miR172及其下游与耐盐性相关的信号转导途径的生物学意义，证明miR172是水稻和小麦耐盐性的正调控因子。更有趣的是，MIR172a和miR172b而不是miR172c或miR172d参与了盐胁迫反应，强调了miR172家族成员的功能分化。进一步的证据揭示了一个新的miR172/IDS1调控模块，它主要通过平衡一组ROS清除基因的表达，在盐胁迫期间作为关键的分子变阻器来维持ROS的动态平衡。我们的发现建立了miRNAs与谷类作物的解毒反应之间的直接分子联系，从而提高了谷类作物的耐盐性。
Salt stress triggers the overdose accumulation of reactive oxygen species (ROS) in crop plants, leading to severe oxidative damage to living tissues. MicroRNAs (miRNAs) act as master regulators orchestrating the stress responsive regulatory networks as well as salt tolerance. However, the fundamental roles of miRNAs in modulating salt tolerance in cereal crops, especially in salt-triggered ROS scavenging remain largely unknown.Through small RNA sequencing, a salt-responsive miRNA, miR172 was identified in rice. Further, by generating the miR172-overexpression or MIR172 gene loss-of-function mutant lines, the biological significance of miR172 and its downstream signaling pathways related to salt tolerance were defined.We demonstrated that miR172 is a positive regulator of salt tolerance in both rice and wheat. More interestingly, miR172a and miR172b, but not miR172c or miR172d are involved in salt stress response, emphasizing the functional differentiation within miR172 family members. Further evidence uncovers a novel miR172/IDS1 regulatory module that functions as a crucial molecular rheostat in maintaining ROS homeostasis during salt stress, mainly through balancing the expression of a group of ROS-scavenging genes.Our findings establish a direct molecular link between miRNAs and detoxification response in cereal crops for improving salt tolerance.