In vitro effects of N-acetylcysteine alone and combined with tigecycline on planktonic cells and biofilms of Acinetobacter baumannii
In vitro effects of N-acetylcysteine alone and combined with tigecycline on planktonic cells and biofilms of Acinetobacter baumannii
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N-乙酰半胱氨酸单用及联合替加环素对鲍曼不动杆菌浮游细胞和生物膜的体外影响
DOI：
10.21037/jtd.2017.11.130
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发表时间：
2018-01-01
影响因子：
2.5
通讯作者：
Zhou, Yanbin
中科院分区：
文献类型：
--
作者：
Feng, Jinlun;Liu, Baomo;Zhou, Yanbin
背景：鲍曼不动杆菌（Acinetobacter baumannii，A.鲍曼不动杆菌）作为一种常见的机会致病菌，具有较强的形成生物被膜的能力，从而导致耐药性和慢性感染。N-乙酰半胱氨酸（NAC）和替加环素（TGC）的组合被证明可以协同抑制生物膜相关的细菌感染，包括耐甲氧西林金黄色葡萄球菌和表皮葡萄球菌。本研究旨在探讨NAC和TGC对A.方法：采用微量肉汤稀释法测定NAC的最低抑菌浓度（MIC）。结晶紫染色法测定生物膜敏感性。用棋盘法测定NAC和TGC对细胞增殖的交互作用。以活细胞计数法评价NAC和TGC对生物膜包埋细菌的联合作用。鲍曼不动杆菌分离株范围为16 - 128 mg/mL。单独的NAC（0.5-128 mg/mL）显著抑制生物膜形成并破坏预先形成的生物膜。NAC和TGC联合使用对嗜热菌的作用表现为部分协同效应（60%）和相加效应（28%）。对于生物膜包埋的细菌，单独用16 mg/mL NAC或单独用2 μ g/mL TGC处理导致显著的杀菌效果（P
Background: Acinetobacter baumannii (A. baumannii), as a common opportunistic pathogen, has strong ability to form biofilms, which has led to drug resistance and chronic infections. The combination of N-acetylcysteine (NAC) and tigecycline (TGC) was demonstrated to synergistically inhibit biofilm-associated bacterial infections, including methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis. The purpose of this study is to investigate the effect of NAC and TGC on planktonic cells and biofilms of A. baumannii.Methods: Minimum inhibitory concentrations (MICs) of NAC were determined by broth microdilution method. Biofilm susceptibility was assessed by crystal violet stain. Interactive effects of NAC and TGC on planktonic cells were determined by checkerboard MIC assay. Viable cell count was used to evaluate the combined effect of NAC and TGC on biofilm-embedded bacteria.Results: MICs of NAC against 25 A. baumannii isolates ranged from 16 to 128 mg/mL. NAC alone (0.5-128 mg/mL) significantly inhibited biofilm formation and disrupted preformed biofilms. The combination of NAC and TGC induced a partial synergistic effect (60%) and additive effect (28%) on planktonic bacteria. For biofilm-embedded bacteria, treatment with 16 mg/mL NAC alone or 2 mu g/mL TGC alone resulted in significant bactericidal effects (P