Transduction and Plasmid Deoxyribonucleic Acid Analysis in a Multiply Antibiotic-Resistant Strain of Staphylococcus epidermidis
Transduction and Plasmid Deoxyribonucleic Acid Analysis in a Multiply Antibiotic-Resistant Strain of Staphylococcus epidermidis
复制标题
表皮葡萄球菌多重抗生素耐药菌株的转导和质粒脱氧核糖核酸分析
DOI：
10.1128/jb.120.2.679-686.1974
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发表时间：
1974
影响因子：
3.2
通讯作者：
F. Kayser
中科院分区：
文献类型：
--
作者：
L. Rosendorf;F. Kayser
对一株多重耐药表皮葡萄球菌进行了遗传分析。旨在显示生物体对抗生素敏感性的逆转的实验，以及紫外线照射噬菌体对抗性标记转导频率的影响的研究表明，氯霉素（cml）、四环素（泰特）和新霉素（neo）抗性的决定因素存在于单独的质粒上，但链霉素标记是染色体。在2 - 6%的四环素抗性转导子中，也观察到cml的共转导。通过使用氯化铯染料密度梯度，然后用中性蔗糖梯度，携带cml，泰特，和neo的质粒可以被分离，并可以确定它们的分子量。四环素质粒显示与受体菌株的隐蔽质粒之一不相容。
A genetic analysis of a multiply antibiotic-resistant strain of Staphylococcus epidermidis was performed. Experiments designed to show reversion of organisms to antibiotic susceptibility, as well as studies of the influence of ultraviolet irradiation of phage on the transduction frequencies of the resistance markers, indicated that determinants of chloramphenicol (cml), tetracycline (tet), and neomycin (neo) resistance are present on separate plasmids, but the streptomycin marker is chromosomal. In 2 to 6% of tetracycline-resistant transductants, co-transduction of cml was also observed. By using CsCl-dye density gradients followed by neutral sucrose gradients, the plasmids carrying cml, tet, and neo could be isolated and their molecular weights could be determined. The tetracycline plasmid is shown to be incompatible with one of the cryptic plasmids of a recipient strain.