Progressive increase of glucose transporter-3 (GLUT-3) expression in estrogen-induced breast carcinogenesis.
Progressive increase of glucose transporter-3 (GLUT-3) expression in estrogen-induced breast carcinogenesis.
复制标题
DOI：
10.1007/s12094-012-0882-3
复制
发表时间：
2013-01
影响因子：
3.4
通讯作者：
Russo, J.
中科院分区：
文献类型：
--
作者：
Kocdor, M. A.;Kocdor, H.;Pereira, J. S.;Vanegas, J. E.;Russo, I. H.;Russo, J.
葡萄糖摄取增加和糖酵解是恶性肿瘤细胞的主要代谢特征。葡萄糖转运蛋白家族(GLUT)以肿瘤特异性的方式促进葡萄糖跨质膜的移动。葡萄糖转运蛋白-1(GLUT-1)、GLUT-3和最近的GLUT-12已在乳腺癌细胞中被发现，并被发现与不良预后有关。此外，已有研究表明雌激素在过量调控中起着关键作用，然而，对乳腺癌发生的阶段特异性过剩调控尚不清楚。在体外-体内渐进性雌激素诱导的乳腺癌模型中，研究了GLUT的表达模式，该模型由四个具有相同遗传背景的细胞系组成。该模型代表了肿瘤发生和发展的不同阶段，MCF-10F为正常阶段，E2细胞为雌激素转化阶段，C5细胞为侵袭期，T4细胞为成瘤阶段。此外，还计数了胶原基质中导管形成和固体团块形成的丢失以及细胞的侵袭力。实时荧光定量聚合酶链式反应显示，17β-雌二醇(E_2)作用于MCF10F后，GLUT1表达下调，在侵袭性细胞(C5)中表达下调，但在肿瘤细胞(T4)中表达无明显变化。C5和T4细胞中GLUT-3的表达水平最高。这些细胞也被发现与导管形成丧失、固体肿块形成和更高的侵袭能力有关，而GLUT-12在C5和T4细胞中下调。雌激素诱导的恶性转化与显著和进行性的GLUT-3表达、进一步阶段的GLUT-1重新表达以及GLUT-12下调有关。
Increased glucose uptake and glycolysis are main metabolic characteristics of malignant cells. A family of glucose transporters (GLUTs) facilitates glucose movement across the plasma membranes in a tumor-specific manner. Glucose transporter-1 (GLUT-1), GLUT-3 and recently GLUT-12, have been previously shown in breast cancer cells and are found to be associated with poor prognosis. In addition, it has been shown that estrogen plays critical roles in GLUT regulation, however, the stage-specific GLUT regulation of mammary carcinogenesis is unclear. GLUT expression patterns were investigated in an in vitro–in vivo progressive, estrogen-induced, mammary carcinogenesis model which consisted of four cell lines, with same genetic background. In this model, different stages of tumor initiation and progression are represented, MCF-10F being the normal stage, E2 cells the transformed stage by estrogen, C5 cells, the invasive stage, and T4 cells the tumorigenic stage. In addition, loss of ductulogenesis and solid mass formation in collagen matrix and invasiveness of the cells were counted. Real time PCR showed that GLUT1 expression was downregulated in MCF10F after treatment with 17β-estradiol (E2), and in the invasive cell type (C5), but not in the tumor cells (T4), which had no changes compared to MCF10F. C5 and T4 cells showed the highest rate of GLUT-3 expression. These cells were also found to be associated with loss of ductulogenesis, solid mass formation and higher invasive capacity, whereas, GLUT-12 was downregulated in C5 and T4 cells. Estrogen-induced malignant transformation is associated with remarkable and progressive GLUT-3 expression, GLUT-1 re-expression at further stages, as well as GLUT-12 downregulation.
登录
查看更多内容
DOI：
10.1111/j.1349-7006.2001.tb01064.x
发表时间：
2001-10
期刊：
Japanese journal of cancer research : Gann
影响因子：
--
作者：
Kurebayashi J;Otsuki T;Moriya T;Sonoo H
通讯作者：
Sonoo H
影响因子：
56.9
作者：
FLIER, JS;MUECKLER, MM;LODISH, HF
通讯作者：
LODISH, HF
影响因子：
11.2
作者：
Liao, Debbie;Corle, Courtney;Johnson, Randall S.
通讯作者：
Johnson, Randall S.
影响因子：
11.5
作者：
Cooper, C;Liu, GY;Watson, PH
通讯作者：
Watson, PH
DOI：
10.1016/j.bbrc.2006.02.094
发表时间：
2006-04-21
影响因子：
3.1
作者：
Li, J;Shi, MX;Zhao, RCH
通讯作者：
Zhao, RCH