Evolution of color vision in pierid butterflies: blue opsin duplication, ommatidial heterogeneity and eye regionalization in Colias erate
Evolution of color vision in pierid butterflies: blue opsin duplication, ommatidial heterogeneity and eye regionalization in Colias erate
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DOI：
10.1007/s00359-009-0418-7
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发表时间：
2009-04-01
影响因子：
2.1
通讯作者：
Arikawa, Kentaro
中科院分区：
文献类型：
--
作者：
Awata, Hiroko;Wakakuwa, Motohiro;Arikawa, Kentaro
本文记录了东方浅黄色云蝶（粉蝶科）眼睛的分子组织。我们克隆了四个编码视色素视蛋白的cDNA，分别对应于一个紫外、两个蓝色和一个长波长吸收视色素。蓝色视觉色素类的复制也发生在另一个粉蝶科物种，粉蝶，这表明蓝色复制是粉蝶科的一个普遍特征。我们通过原位杂交定位了视蛋白的mRNA在大肠杆菌视网膜。在9个感光细胞的小眼，R1-9，我们发现，R3-8表达的长波长类mRNA在所有的小眼。R1和R2以三种固定的组合表达短波长视蛋白的mRNA，对应于三种类型的小眼。虽然复制的蓝色视蛋白在粉蝶分别表达在两个子集的R1-2光感受器，一个蓝色敏感和另一个紫色敏感，那些大肠杆菌似乎总是共表达。
This paper documents the molecular organization of the eye of the Eastern Pale Clouded Yellow butterfly, Colias erate (Pieridae). We cloned four cDNAs encoding visual pigment opsins, corresponding to one ultraviolet, two blue and one long wavelength-absorbing visual pigments. Duplication of the blue visual pigment class occurs also in another pierid species, Pieris rapae, suggesting that blue duplication is a general feature in the family Pieridae. We localized the opsin mRNAs in the Colias retina by in situ hybridization. Among the nine photoreceptor cells in an ommatidium, R1-9, we found that R3-8 expressed the long wavelength class mRNA in all ommatidia. R1 and R2 expressed mRNAs of the short wavelength opsins in three fixed combinations, corresponding to three types of ommatidia. While the duplicated blue opsins in Pieris are separately expressed in two subsets of R1-2 photoreceptors, one blue sensitive and another violet sensitive, those of Colias appear to be always coexpressed.