Lah is a transmembrane protein and requires Spa10 for stable positioning of Woronin bodies at the septal pore of Aspergillus fumigatus.
Lah is a transmembrane protein and requires Spa10 for stable positioning of Woronin bodies at the septal pore of Aspergillus fumigatus.
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DOI：
10.1038/srep44179
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发表时间：
2017-03-10
影响因子：
4.6
通讯作者：
Ebel F
中科院分区：
文献类型：
--
作者：
Leonhardt Y;Kakoschke SC;Wagener J;Ebel F
Woronin体是一种特殊的真菌特异性细胞器，能够在损伤后立即关闭隔膜孔，以保护菌丝免受过度细胞质出血。在大多数子囊菌中，Woronin体被所谓的Lah蛋白质束缚在隔膜孔处。使用致病性霉菌烟曲霉作为模式生物，我们表明，C-末端的288个氨基酸的拉（LahC 288）结合的隔膜孔的边缘。LahC 288基本上由跨膜区和推定的胞外结构域组成，这两者都是靶向隔膜所需的。在一个A。在具有隔膜形成的严重缺陷的烟曲霉rho 4缺失突变体中，LahC 288被募集到侧膜中或侧膜处的点状结构。这表明LahC在Rho 4开始支配分隔过程之前被招募。因此，我们发现，在野生型菌丝Lah的约束前出现的横壁，从而使Woronin机构在网站上的新形成的隔膜拴系。最后，我们确定了Spa 10，最近描述的中隔孔相关蛋白家族的成员，作为第一个蛋白质，直接或间接地与LahC相互作用，以允许一个稳定的定位Woronin机构在成熟的隔膜。
Woronin bodies are specialized, fungal-specific organelles that enable an immediate closure of septal pores after injury to protect hyphae from excessive cytoplasmic bleeding. In most Ascomycetes, Woronin bodies are tethered at the septal pore by so-called Lah proteins. Using the pathogenic mold Aspergillus fumigatus as a model organism, we show that the C-terminal 288 amino acids of Lah (LahC288) bind to the rim of the septal pore. LahC288 essentially consists of a membrane spanning region and a putative extracellular domain, which are both required for the targeting to the septum. In an A. fumigatus rho4 deletion mutant that has a severe defect in septum formation, LahC288 is recruited to spot-like structures in or at the lateral membrane. This suggests that LahC is recruited before Rho4 starts to govern the septation process. Accordingly, we found that in wild type hyphae Lah is bound before a cross-wall emerges and thus enables a tethering of Woronin bodies at the site of the newly formed septum. Finally, we identified Spa10, a member of a recently described family of septal pore-associated proteins, as a first protein that directly or indirectly interacts with LahC to allow a stable positioning of Woronin bodies at the mature septum.