Ammonia induce lung tissue injury in broilers by activating NLRP3 inflammasome via Escherichia/Shigella.
Ammonia induce lung tissue injury in broilers by activating NLRP3 inflammasome via Escherichia/Shigella.
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DOI：
10.1016/j.psj.2020.03.019
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发表时间：
2020-07
期刊：
影响因子：
4.4
通讯作者：
Zhang MH
中科院分区：
文献类型：
--
作者：
Liu QX;Zhou Y;Li XM;Ma DD;Xing S;Feng JH;Zhang MH
呼吸道疾病与大气污染密切相关。氨是大气环境中的有害污染物之一，对人畜呼吸道健康有很大威胁，但其致病机制尚不清楚。本研究以肉鸡肺组织为模型，通过呼吸道菌群、NLRP3炎性体和炎症因子之间的关系，研究高氨对呼吸道疾病的影响。为此，我们验证了氨暴露下肺组织炎症的发生，并通过16S rDNA测序检测了肺组织微生物成分。检测NLRP3和caspase-1 mRNA的相对表达量以及IL-1β和IL-6的含量。7 d氨暴露后，肺组织中变形菌门、埃希氏菌属/志贺氏菌属比例显著升高，NLRP3和caspase-1 mRNA表达水平显著升高，肺组织和血清中IL-1β含量高于对照组。综上所述，高氨诱导肺组织炎症是通过增加埃希氏菌/志贺氏菌比例，激活NLRP3炎性体，促进IL-1β释放来实现的。研究结果可为氨污染引起的人畜呼吸道疾病的防治提供参考。
Respiratory tract diseases are closely related to atmosphere pollution. Ammonia is one of the harmful pollutants in the atmosphere environment, which has a great threat to human and animal respiratory tract health, but the mechanism of causing diseases is not clear. In this study, broiler lung tissue was used as a model to study the effect of high ammonia on respiratory tract diseases through the relationship between respiratory microflora, NLRP3 inflammasome, and inflammatory factors. For this, we validated the occurrence of lung tissue inflammation under ammonia exposure and detected the lung tissue microbial constituent by 16S rDNA sequencing. Moreover, the relative expression levels of NLRP3 and caspase-1 mRNA and the content of IL-1β and IL-6 were measured. After 7-D ammonia exposure, the proportion of the phylum Proteobacteria and the genus Escherichia/Shigella in lung tissue was significantly increased, the expression levels of NLRP3 and caspase-1 mRNA were significantly increased, and the content of IL-1β in lung tissue and serum was higher than that in the control group. In conclusion, high ammonia induced lung tissue inflammation via increasing the proportion of Escherichia/Shigella, activating NLRP3 inflammasome, and promoting IL-1β release. These findings provided a reference for the prevention and control of respiratory tract diseases in humans and animals caused by ammonia pollution.
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DOI：
10.3390/ijerph7010089
发表时间：
2010-01
影响因子：
--
作者：
Lutful Kabir SM
通讯作者：
Lutful Kabir SM
影响因子：
15.3
作者：
Ichinohe, Takeshi;Lee, Heung Kyu;Ogura, Yasunori;Flavell, Richard;Iwasaki, Akiko
通讯作者：
Iwasaki, Akiko
影响因子：
30.5
作者：
通讯作者：
--
影响因子：
5.1
作者：
Liu Z;Zhao H;Liu W;Li T;Wang Y;Zhao M
通讯作者：
Zhao M
影响因子：
32.4
作者：
Allen, Irving C.;Scull, Margaret A.;Moore, Chris B.;Holl, Eda K.;McElvania-TeKippe, Erin;Taxman, Debra J.;Guthrie, Elizabeth H.;Pickles, Raymond J.;Ting, Jenny P. -Y.
通讯作者：
Ting, Jenny P. -Y.