Protein level of apolipoprotein E increased in human hepatocellular carcinoma.
Protein level of apolipoprotein E increased in human hepatocellular carcinoma.
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DOI：
10.3892/ijo.28.3.625
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发表时间：
2006-03
影响因子：
5.2
通讯作者：
Yuichirou Yokoyama;Y. Kuramitsu;M. Takashima;N. Iizuka;S. Terai;M. Oka;K. Nakamura;K. Okita;I. Sakaida
中科院分区：
文献类型：
--
作者：
Yuichirou Yokoyama;Y. Kuramitsu;M. Takashima;N. Iizuka;S. Terai;M. Oka;K. Nakamura;K. Okita;I. Sakaida
许多报告表明，肝脂肪变性会导致肝细胞癌（HCC），包括丙型肝炎病毒或非酒精性脂肪性肝炎。通过免疫印迹对 HCC 患者的肿瘤组织进行蛋白质组学研究，重点关注 apoA1、apoB100 和 apoE 的载脂蛋白 (apo)。虽然无法统计显示apoA1或apoB100的显着变化，但免疫印迹显示88%的患者肿瘤组织中apoE蛋白水平升高，而apoE基因表达和血清水平未升高。这些结果表明 apoE 因分泌受损而积累。此外，二维电泳的免疫印迹分析表明，HCC 肿瘤组织中唾液酸化形式的 apoE 也很可能增加。肿瘤组织中的 ApoE 水平经常升高，可能是 HCC 的良好组织学标志物。
Many reports suggest that hepatic steatosis leads to hepatocellular carcinoma (HCC), including hepatitis C virus or non-alcoholic steatohepatitis. Proteomic study of tumor tissues from HCC patients, focusing on apolipoprotein (apo) of apoA1, apoB100 and apoE, was performed by immunoblotting. Although the significant changes of apoA1 or apoB100 could not be shown statistically, the immunoblotting showed the increase in protein level of apoE in the tumor tissues of 88% of patients without increase of apoE gene expression and serum level. These results suggest the accumulation of apoE by impaired secretion. Moreover, immunoblot analysis on two-dimensional electrophoresis showed a strong possibility that sialylated forms of apoE also were increased in tumorous tissues of HCC. ApoE level in tumorous tissues is frequently elevated and may be a good histological marker for HCC.