Tickling increases dopamine release in the nucleus accumbens and 50 kHz ultrasonic vocalizations in adolescent rats
Tickling increases dopamine release in the nucleus accumbens and 50 kHz ultrasonic vocalizations in adolescent rats
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DOI：
10.1097/wnr.0b013e32835edbfa
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发表时间：
2013-03-27
期刊：
影响因子：
1.7
通讯作者：
Kurosawa, Mieko
中科院分区：
文献类型：
--
作者：
Hori, Miyo;Shimoju, Rie;Kurosawa, Mieko
青春期的老鼠在打闹玩耍或受到挠痒刺激时，会发出50千赫的超声波，这是积极情绪的标志。挠痒时发出的50 kHz超声声音可能是由伏隔核释放多巴胺介导的；然而，没有直接证据支持这一假设。本研究旨在阐明青春期大鼠的玩耍行为（挠痒）是否可以通过50 kHz的快乐超声发声触发伏隔核释放多巴胺。将挠痒刺激与轻触刺激作为一种可辨别的刺激进行了比较。我们研究了35-40天大的大鼠，这相当于青春期的中期。痒刺激5分钟显著增加伏隔核多巴胺释放（刺激前控制值的118 +/- 7%）。相反，轻触刺激5分钟没有显著改变多巴胺释放。此外，在挠痒刺激时发出50 kHz的超声波，而在轻触刺激时则没有。此外，将SCH23390 （D1受体拮抗剂）和raclopride （D2/D3受体拮抗剂）直接应用于伏隔核，可显著阻断挠痒诱导的50 kHz超声发声。我们的研究表明，青春期大鼠的挠痒刺激增加了伏隔核的多巴胺释放，导致产生50 kHz的超声波发声。神经报告24:41 -245 (C) 2013 Wolters Kluwer Health垂直bar Lippincott Williams & Wilkins。
Adolescent rats emit 50 kHz ultrasonic vocalizations, a marker of positive emotion, during rough-and-tumble play or on tickling stimulation. The emission of 50 kHz ultrasonic vocalizations in response to tickling is suggested to be mediated by dopamine release in the nucleus accumbens; however, there is no direct evidence supporting this hypothesis. The present study aimed to elucidate whether play behavior (tickling) in adolescent rats can trigger dopamine release in the nucleus accumbens with hedonic 50 kHz ultrasonic vocalizations. The effect of tickling stimulation was compared with light-touch stimulation, as a discernible stimulus. We examined 35-40-day-old rats, which corresponds to the period of midadolescence. Tickling stimulation for 5 min significantly increased dopamine release in the nucleus accumbens (118 +/- 7% of the prestimulus control value). Conversely, light-touch stimulation for 5 min did not significantly change dopamine release. In addition, 50 kHz ultrasonic vocalizations were emitted during tickling stimulation but not during light-touch stimulation. Further, tickling-induced 50 kHz ultrasonic vocalizations were significantly blocked by the direct application of SCH23390 (D1 receptor antagonist) and raclopride (D2/D3 receptor antagonist) into the nucleus accumbens. Our study demonstrates that tickling stimulation in adolescent rats increases dopamine release in the nucleus accumbens, leading to the generation of 50 kHz ultrasonic vocalizations. NeuroReport 24:241-245 (C) 2013 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.