Molecular cloning, expression, and chromosomal localization of a ubiquitously expressed human 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene (PFKFB3)
Molecular cloning, expression, and chromosomal localization of a ubiquitously expressed human 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene (PFKFB3)
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DOI：
10.1159/000015181
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发表时间：
1998-01-01
期刊：
影响因子：
--
通讯作者：
Bartrons, R
中科院分区：
文献类型：
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作者：
Manzano, A;Rosa, JL;Bartrons, R
我们从人胎脑cDNA文库中克隆了一个人6-磷酸果糖-2-激酶/果糖2，6-二磷酸酶基因（PFKFB 3）。荧光原位杂交结果显示该基因定位于10 p15->p14。整个cDNA（4，322 bp）编码520个氨基酸残基的多肽（分子量，59.571 kDa）。结构分析表明，存在的激酶结构域位于氨基末端和双磷酸酶结构域的羧基末端，以前描述的6-磷酸果糖-2-激酶/果糖2，6-双磷酸酶同工酶的特征。此外，cAMP依赖性蛋白激酶的磷酸化位点被发现在羧基末端。北方印迹分析表明，存在一个独特的4.8 kb的mRNA表达在不同的组织研究。在哺乳动物COS-I细胞中，该cDNA驱动活性同工酶的表达。总之，这些结果鉴定了编码普遍表达的人6-磷酸果糖-2-激酶/果糖2，6-二磷酸酶同工酶的基因的存在。
We report the identification of a human 6-phosphofructo-2-kinase/fnrctose 2,6-bisphosphatase gene (PFKFB3) isolated from a human fetal brain cDNA library. The gene was localized to 10p15-->p14 by fluorescence in situ hybridization. The entire cDNA (4,322 bp) codes for a polypeptide of 520 amino acid residues (molecular weight, 59.571 kDa). Structural analysis showed the presence of a kinase domain located at the amino terminus and a bisphosphatase domain at the carboxy terminus, characteristic of previously described 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase isozymes. In addition, a phosphorylation site for cAMP-dependent protein kinase was found at the carboxy terminus. Northern blot analysis showed the presence of a unique 4.8-kb mRNA expressed in the different tissues studied. In mammalian COS-I cells, this cDNA drives the expression of an active isozyme. Taken together, these results identify the presence of a gene coding for a human 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase isozyme which is ubiquitously expressed.