Protosappanin A, an Immunosuppressive Constituent From a Chinese Herb, Prolongs Graft Survival and Attenuates Acute Rejection in Rat Heart Allografts
Protosappanin A, an Immunosuppressive Constituent From a Chinese Herb, Prolongs Graft Survival and Attenuates Acute Rejection in Rat Heart Allografts
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DOI：
10.1016/j.transproceed.2008.06.097
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发表时间：
2008-12-01
影响因子：
0.9
通讯作者：
Yu, B.
中科院分区：
文献类型：
--
作者：
Wu, J.;Hou, J. B.;Yu, B.
目标。苏木是东医常用的一种止痛、抗炎药物，对大鼠心脏移植急性排斥反应具有免疫抑制作用。本研究旨在探讨苏木的主要成分之一--原突触素A在大鼠心脏移植中的作用。从苏木乙醇提取物中分离得到原皂苷A，经波谱分析鉴定为原皂苷A。所有组均为Wistar供体，接受SD受体，包括大剂量(25 mg/kg)或小剂量(5 mg/kg)的Protosappanin A加环孢素A(10 mg/kg)。另设对照组。术后第2天给药，观察移植物存活情况和心脏病理变化。术后第7天采血测定CD4+/CD8+T细胞比值。逆转录-聚合酶链式反应(RT-PCR)检测移植物穿孔素和颗粒酶B基因的表达水平。原阿帕菌素A或环孢素可显著延长同种异体心脏移植物存活时间(P<；.01)，减轻心肌病理损害(P<；.01)，降低CD4+/CD8+比值(P<；.05)，抑制穿孔素和颗粒酶B基因的表达(P<；.05)。这些发现表明，原激动素A通过显著减轻急性排斥反应延长了同种异体心脏移植物的存活时间。
Objective. Caesalpinia sappan L., which has been used in oriental medicine as an analgesic and antiinflammatory agent, has shown immunosuppressive activity on acute rejection on rat heart allografts. The present study was designed to investigate the potency of protosappanin A, one major ingredient of C sappan L., in rat heart transplantation.Materials and Methods. Protosappanin A isolated from an ethanol extract of C sappan L. was identified by wave spectrum. All groups consisted of Wistar donors into Sprague-Dawley (SD) recipients, including large (25 mg/kg) or small (5 mg/kg) doses of protosappanin A plus cyclosporine (10 mg/kg). A control group was used. Animals were given the drugs on postoperative day 2. We observed graft survival and pathologic conditions of the hearts. Blood samples were obtained on postoperative day 7 to measure the CD4+/CD8+ T-cell ratio. Graft perforin and granzyme B mRNA expression levels were examined with reverse transcription-polymerase chain reaction (RT-PCR) methods.Results. Protosappanin A or cyclosporine significantly prolonged heart allograft survival (P < .01), alleviated myocardial pathologic damages (P < .01), decreased the CD4+/CD8+ ratio (P < .05), and inhibited perforin and granzyme B mRNA expressions in the graft (P < .05).Conclusions. These findings demonstrated that protosappanin A prolonged heart allograft survival by significantly attenuating acute rejection.