High Spatial-Resolution Matrix-Assisted Laser Desorption/Ionization-Ion Trap-Time-of-Flight Tandem Mass Spectrometry Imaging for Depicting Longitudinal and Transverse Distribution of Drugs Incorporated into Hair
High Spatial-Resolution Matrix-Assisted Laser Desorption/Ionization-Ion Trap-Time-of-Flight Tandem Mass Spectrometry Imaging for Depicting Longitudinal and Transverse Distribution of Drugs Incorporated into Hair
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DOI：
10.1021/acs.analchem.9b05401
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发表时间：
2020-04-21
影响因子：
7.4
通讯作者：
Katagi, Munehiro
中科院分区：
文献类型：
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作者：
Kamata, Tooru;Shima, Noriaki;Katagi, Munehiro
本研究的目的是实现高空间分辨率的串联质谱（MS/MS）成像描绘药物在头发中的纵向和横向分布，这可以提供必要的信息，正确解释头发测试结果，包括药物掺入头发的机制。获得并分析了两种类型的头发样品：使用者的头发，从服用含有甲氧那明（MOP）的非处方药的志愿者中取样，MOP是甲基苯丙胺的非管制类似物;浸泡头发，通过将空白头发浸泡在MOP溶液中制备。单根毛干的纵向和横向切片通过使用定制的切片机进行冷冻切片来完成。α-氰基-4-羟基肉桂酸的气相沉积提供了最精细的基质层（分辨率
This study aims to achieve high spatial-resolution tandem mass spectrometry (MS/MS) imaging for depicting longitudinal and transverse distribution of drugs in hair, which can provide indispensable information for the proper interpretation of hair test results, including the mechanism of drug incorporation into hair. Two types of hair samples were obtained and analyzed: User's Hair, sampled from a volunteer who took an over-the-counter medicine containing methoxyphenamine (MOP), a nonregulated analogue of methamphetamine; and Soaked Hair, prepared by soaking blank hair in MOP solution. Longitudinal and transverse-sectioning of single hair shafts was accomplished by freeze-sectioning using customized microtomes. Vapor deposition of alpha-cyano-4-hydroxycinnamic acid provided the finest matrix layer (resolution