Stable germline transformation of the malaria mosquito Anopheles stephensi
Stable germline transformation of the malaria mosquito Anopheles stephensi
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DOI：
10.1038/35016096
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发表时间：
2000-06-22
期刊：
影响因子：
64.8
通讯作者：
Crisanti, A
中科院分区：
文献类型：
--
作者：
Catteruccia, F;Nolan, T;Crisanti, A
按蚊是人类疟疾的必然媒介，疟疾是一种影响生活在热带和亚热带国家的数亿人的传染病。由于缺乏合适的基因转移技术，这些蚊子的生理学，包括载体和疟疾寄生虫之间的分子相互作用的分子遗传学分析受到阻碍。在这里，我们表明，一个转座子，基于Minos元件（1）和携带外源DNA，可以有效和稳定地整合到人类疟疾载体斯氏按蚊的生殖系，通过转座酶介导的过程。
Anopheline mosquito species are obligatory vectors for human malaria, an infectious disease that affects hundreds of millions of people living in tropical and subtropical countries. The lack of a suitable gene transfer technology for these mosquitoes has hampered the molecular genetic analysis of their physiology, including the molecular interactions between the vector and the malaria parasite. Here we show that a transposon, based on the Minos element(1) and bearing exogenous DNA, can integrate efficiently and stably into the germ line of the human malaria vector Anopheles stephensi, through a transposase-mediated process.