SNHG7 accelerates cell migration and invasion through regulating miR-34a-Snail-EMT axis in gastric cancer
SNHG7 accelerates cell migration and invasion through regulating miR-34a-Snail-EMT axis in gastric cancer
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DOI：
10.1080/15384101.2019.1699753
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发表时间：
2019-12-09
期刊：
影响因子：
4.3
通讯作者：
Chen, Kai
中科院分区：
文献类型：
--
作者：
Zhang, Yangmei;Yuan, Yuan;Chen, Kai
小核仁 RNA 宿主基因 7 (SNHG7) 是大多数人类癌症中新近发现的致癌长链非编码 RNA (lncRNA)。在胃癌中，SNHG7被认为通过下调P15和P16的表达来增强细胞增殖并抑制细胞凋亡，但SNHG7对胃癌细胞迁移和侵袭的影响仍不清楚。在我们的研究中，我们旨在评估SNHG7表达与临床和病理特征之间的关系，并探讨SNHG7对胃癌细胞迁移和侵袭的影响。本研究发现胃癌组织和细胞系中SNHG7的表达水平显着高于正常癌旁组织和胃粘膜上皮细胞。此外，SNHG7的高表达与胃癌患者的TNM分期、浸润深度、淋巴结转移和远处转移呈正相关。此外，多变量Cox比例风险分析进一步显示SNHG7高表达是胃癌患者总生存期的独立不良预后因素。体外研究表明，SNHG7直接与miR-34a结合并负向调节miR-34a的表达，SNHG7通过抑制miR-34a-Snail-EMT轴增强胃癌细胞的迁移和侵袭。总之，SNHG7在胃癌中作为致癌lncRNA发挥作用，可能成为胃癌患者的潜在治疗靶点。
Small nucleolar RNA host gene 7 (SNHG7) is a newly recognized oncogenic Long non-coding RNA (lncRNA) in most human cancers. In gastric cancer, SNHG7 has been suggested to enhance cell proliferation and suppressed apoptosis through down-regulating P15 and P16 expression, but the effect of SNHG7 on gastric cancer cell migration and invasion was still unknown. In our study, we aimed to estimate the relationship between SNHG7 expression and clinical and pathological characteristics, and explore the effect of SNHG7 on gastric cancer cell migration and invasion. In our study, the levels of SNHG7 expression in gastric cancer tissues and cell lines were severally higher than in normal adjacent tissues and gastric mucosal epithelial cells. Moreover, high SNHG7 expression was positively correlated with TNM stage, depth of invasion, lymph-node metastasis and distant metastasis in gastric cancer patients. Furthermore, the multivariate Cox proportional hazard analysis further showed high SNHG7 expression was an independent poor prognostic factor for overall survival in gastric cancer patients. The studies in vitro revealed that SNHG7 directly binds to miR-34a and negatively regulates miR-34a expression, and SNHG7 enhances gastric cancer cell migration and invasion through suppressing miR-34a-Snail-EMT axis. In conclusion, SNHG7 functions as oncogenic lncRNA in gastric cancer and may be a potential therapeutic target for gastric cancer patients.