Identification and expression profile of a novel alternative splicing of Pax7 in chick skeletal muscle.
Identification and expression profile of a novel alternative splicing of Pax7 in chick skeletal muscle.
复制标题
鸡骨骼肌中 Pax7 的新型选择性剪接的鉴定和表达谱。
DOI：
10.3382/ps.2007-00479
复制
发表时间：
2008
期刊：
影响因子：
4.4
通讯作者：
Ning Li
中科院分区：
文献类型：
--
作者：
C. Mao;Xiaoxiang Hu;Ning Li
Pax 7是骨骼肌肌细胞发生所必需的。Pax 7在人和小鼠中存在选择性剪接形式。在这项研究中，我们确定了一个新的选择性剪接鸡Pax 7。我们将其命名为Pax 7 -2，并定位于鸡成肌细胞的细胞核。在Pax 7 -2 mRNA中，鸡Pax 7基因的外显子8被剪切掉。这导致Pax 7 -2蛋白的羧基端与Pax 7蛋白相比缺失了22个氨基酸。荧光素酶分析表明鸡Pax 7具有反式激活蛋白的功能，Pax 7 -2缺失的22个氨基酸可能属于Pax 7的反式激活结构域。Pax 7 -2丧失反式激活能力。检测不同发育时期鸡胸肌中Pax 7 -2的表达水平，发现Pax 7 -2在d-12鸡胚中表达最高。不同发育阶段鸡胸大肌中Pax 7和Pax-2的表达水平在整个研究期间具有相似的趋势，但其表达水平的变化有所不同。随着雏鸡的长大，Pax 7和Pax 7 -2的表达水平要低得多。
Pax7 is essential for skeletal muscle myogenesis. The alternative splicing forms of Pax7 were found in human and mouse. In this study, we identified a new alternative splicing of chick Pax7. We named it Pax7-2 and localized it in the nucleus of chick myoblast. In Pax7-2 mRNA, exon 8 of chick Pax7 gene was spliced out. That led to a 22-amino acid deletion in the COOH terminal of Pax7-2 protein compared with Pax7 protein. Luciferase assays demonstrated that chick Pax7 could act as a transactivator and the deleted 22 amino acids in Pax7-2 may belong to the transactivation domain of Pax7. Pax7-2 lost transactivation ability. We detected the expression levels of Pax7-2 in chick pectoralis muscles at different developmental stages and found that the expression of Pax7-2 was at its peak in d-12 chick embryos. The expression levels of Pax7 and Pax-2 in chick pectoralis muscles at different developmental stages had a similar trend across the period under study, although the changes of their expression levels were different. As chicks grew up, Pax7 and Pax7-2 were expressed at much lower levels.
影响因子：
4.6
作者：
A. Borycki;Jun Li;F. Jin;C. Emerson;Jonathan A. Epstein
通讯作者：
A. Borycki;Jun Li;F. Jin;C. Emerson;Jonathan A. Epstein