CD133+cell content does not influence recovery time after hematopoietic stem cell transplantation
CD133+cell content does not influence recovery time after hematopoietic stem cell transplantation
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DOI：
10.1111/j.1537-2995.2009.02292.x
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发表时间：
2009-11-01
期刊：
影响因子：
2.9
通讯作者：
Gomez-Almaguer, David
中科院分区：
文献类型：
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作者：
Jaime-Perez, Jose Carlos;Guillermo-Villanueva, Victor A.;Gomez-Almaguer, David
背景：输注足够剂量的CD 34+单核造血干细胞（HSCs）是确保造血移植成功的最重要变量。CD 133 + HSC构成具有较高分化潜能的CD 34+亚群。粒细胞集落刺激因子（G-CSF）动员的CD 133+造血干细胞的数量，在造血移植和它的关系，需要恢复hematopoiesis的天数determined.Study设计和方法：38例恶性血液病患者接受自体（n = 15）或异基因（n = 23）造血干细胞移植进行了前瞻性评价。G-CSF以10 μ g/kg/天给药5天。在第5天通过白细胞去除术从外周血回收造血祖细胞。CD 34+和CD 133 +/CD 34+细胞群通过流式细胞术进行定量;血液学恢复的天数被记录下来。结果：回收并移植的中位剂量为4.56 × 106/kg CD 34 + HSC（范围，1.35 × 106-14.6 × 106）;在这些移植的细胞中，中位3.25 × 106也是CD 133+（范围，1.25 × 106-14.3 × 106）。在自体组中，血小板（PLT）计数达到20 x 109/L或更高的中位天数为12天，中性粒细胞计数达到0.5 x 109/L或更高的中位天数为15天;在同种异体组中，分别需要13天和16天（p > 0.05）。平均76.5%的G-CSF动员的CD 34 + HSC共表达CD 133+抗原（范围，23.1-97.9）。结论：移植物中较高数量的CD 133 +/CD 34 + HSC与同种异体或自体受体中较短的中性粒细胞或PLT恢复时间无明显相关性。
BACKGROUND:Infusion of an adequate dose of CD34+ mononuclear hematopoietic stem cells (HSCs) is the single most important variable to assure success in hematopoietic grafting. CD133+ HSCs constitute the CD34+ subgroup with higher differentiation potential. The number of granulocyte-colony-stimulating factor (G-CSF)-mobilized CD133+ HSCs administered during hematopoietic grafting and its relationship with the number of days needed to regain hematopoiesis was determined.STUDY DESIGN AND METHODS:Thirty-eight patients with malignant hematologic diseases who received an autologous (n = 15) or allogeneic (n = 23) HSC transplant were prospectively evaluated. G-CSF was administered for 5 days at 10 mu g/kg/day. Hematopoietic progenitors were recovered from peripheral blood on day 5 by leukopheresis. CD34+ and CD133+/CD34+ cell populations were quantified by flow cytometry; the number of days to hematologic recovery was documented.RESULTS:A median dose of 4.56 x 106/kg CD34+ HSCs (range, 1.35 x 106-14.6 x 106) was recovered and transplanted; of these grafted cells, a median 3.25 x 106 were also CD133+ (range, 1.25 x 106-14.3 x 106). In the autologous group, the median number of days to reach a platelet (PLT) count of 20 x 109/L or greater was 12, and 15 days to obtain a neutrophil count of 0.5 x 109/L or greater; in the allogeneic group 13 and 16 days, respectively, were required (p > 0.05). A median 76.5% of G-CSF-mobilized CD34+ HSCs coexpressed the CD133+ antigen (range, 23.1-97.9).CONCLUSIONS:A higher number of CD133+/CD34+ HSCs in the graft was not clearly associated with a shorter neutrophil or PLT recovery time in either allogeneic or autologous recipients.