Evidence for incorporation of bone marrow-derived endothelial cells into perfused blood vessels in tumors
Evidence for incorporation of bone marrow-derived endothelial cells into perfused blood vessels in tumors
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DOI：
10.1182/blood-2005-08-3210
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发表时间：
2006-04-01
期刊：
影响因子：
20.3
通讯作者：
Jain, RK
中科院分区：
文献类型：
--
作者：
Duda, DG;Cohen, KS;Jain, RK
最近的研究表明，骨髓对肿瘤新生血管的细胞贡献是高度复杂的。在这种情况下，骨髓来源的细胞作为真正的内皮（非造血）细胞进入灌注的肿瘤血管或出生后形成的任何新血管（血管发生）的程度尚不清楚。为此，我们开发了模型来表征局部血管来源和骨髓来源的内皮细胞（BMD-EC）。然后，我们根据一组内皮标志物和形态学表征BMD-ECs。最后，我们使用移植的以及自发的原发性和转移性肿瘤模型量化了它们对肿瘤中灌注血管的贡献。我们证明了BMD-ECs在灌注的肿瘤血管中结合，并且这种贡献随器官部位和小鼠品系而变化。
Recent studies have demonstrated that the cellular contribution of the bone marrow to tumor neovascularization is highly complex. In this context, the extent to which bone marrow-derived cells incorporate as bona fide endothelial (nonhematopoietic) cells into perfused tumor vessels, or any new vessels formed postnatally (vasculogenesis), is unclear. To this end, we developed models to characterize local vessel-derived and bone marrow-derived endothelial cells (BMD-ECs). Then, we characterized the BMD-ECs based on a set of endothelial markers and morphology. Finally, we quantified their contribution to perfused blood vessels in tumors using transplanted as well as spontaneous primary and metastatic tumor models. We demonstrate that BMD-ECs incorporate in perfused tumor vessels, and that this contribution varies with organ site and mouse strain.