p0071 interacts with E-cadherin in the cytoplasm so as to promote the invasion and metastasis of non-small cell lung cancer
p0071 interacts with E-cadherin in the cytoplasm so as to promote the invasion and metastasis of non-small cell lung cancer
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p0071与细胞质中的E-cadherin相互作用促进非小细胞肺癌的侵袭和转移
DOI：
10.1002/mc.22734
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发表时间：
2018
影响因子：
4.6
通讯作者：
Wang Enhua
中科院分区：
文献类型：
--
作者：
Zhao Huanyu;Zhang Di;Yang Lianhe;Wang Enhua
p0071作为p120-catenin（p120 ctn）亚家族的一员，在肿瘤中的研究非常有限。我们证明了p0071在非小细胞肺癌（NSCLC）中的临床病理学意义，以及E-钙粘蛋白。采用免疫共沉淀法检测A549和SPC细胞中p0071与E-钙粘蛋白的相互作用（E-钙粘蛋白主要在这些细胞的细胞质中表达）。p0071细胞质表达在这些细胞中被siRNA敲低，并测量这种对RhoA活性和细胞侵袭和迁移能力的影响。p0071在肿瘤细胞胞浆中的过度表达与淋巴结转移酶和NSCLC的预后不良有关。p0071和E‐cadherin均异常表达（胞浆表达）的患者生存期显著短于无两种异常表达的患者（P< 0.05）。NSCLC组织中p0071和E‐cadherin的细胞质过表达之间存在显著相关性。p0071在A549和SPC细胞系的细胞质中与E‐钙粘蛋白相互作用。siRNA-p0071处理抑制了NSCLC细胞的侵袭和迁移能力。上述结果证实p0071与细胞质中的E‐cadherin相互作用，从而促进NSCLC的侵袭和转移。
As a member of the p120‐catenin (p120ctn) subfamily, the p0071 study in tumor is very limited. We demonstrated the clinicopathological significance of p0071 in non‐small cell lung cancer (NSCLC), as well as E‐cadherin. Co‐immunoprecipitation was used to detect the interaction of p0071 with E‐cadherin in A549 and SPC cells (E‐cadherin is mainly expressed in the cytoplasm of these cells). p0071 cytoplasmic expression was knocked down by siRNA in these cells and this effect on the RhoA activity and cell invasion and migration ability were measured. p0071 overexpression in the cytoplasm of tumor cell was correlated with lymphatic metastase and poor prognosis of NSCLC. The patients with both abnormal expression of p0071 and E‐cadherin (cytoplasmic expression) had a statistically significant shorter survival than the patients without both abnormal expression (P< 0.05). There is a significant correlation between cytoplasmic overexpression of p0071 and E‐cadherin in NSCLC tissues. p0071 interacted with E‐cadherin in the cytoplasm of A549 and SPC cell lines. Treatment with siRNA‐p0071 inhibited the invasion and migration ability of NSCLC cells. Above results confirmed that p0071 interacted with E‐cadherin in the cytoplasm so as to promote the invasion and metastasis of NSCLC.